长期大剂量服用双膦酸盐不会导致骨骼过度矿化。

[Long-term and high-dose bisphosphonate administration causes no bone over-mineralization].

作者信息

Liu Chao, Cao Yong-ping, Yang Xin, Mashiba Tasuku, Mori Satoshi

机构信息

Department of Orthopedics, Peking University First Hospital, Beijing, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2009 Nov 17;89(42):2968-71.

PMID:20137705

Abstract

OBJECTIVE

To evaluate the effects of long-term bisphosphonate administration (incadronate) upon the mean degree of secondary bone mineralization.

METHODS

Thirty adult beagles were divided randomly into three groups of CNT, YML and YMH based upon their body weights (5 males and 5 females in each group). Animals in CNT were orally given lactose 12 mg x kg(-1) x d(-1) while those in YML and YMH were orally given incadronate disodium at doses of 0.3 mg x kg(-1) x d(-1) and 0.6 mg x kg(-1) x d(-1) respectively. The dosing procedure lasted for three years. Prior to sacrifices, all animals were double-labeled intravenously with oxytetracycline hydrochloride (20 mg x kg(-1)). After necropsy, the left 9th ribs were excised for evaluation.

RESULTS

Histomorphometry showed that activation frequency in YML and YMH [(4.93 +/- 0.92)/mm(2) and (2.50 +/- 0.78)/mm(2)] were significantly lower than that in CNT (7.83 +/- 0.96)/mm(2) per year. The mean degree of mineralization in bone in YML and YMH (1.40 +/- 0.12, 1.48 +/- 0.09) were significantly higher than CNT (1.07 +/- 0.06). The increments were 31% and 38% respectively.

CONCLUSION

Long-term and high-dose bisphosphonate administration significantly suppresses bone turnover so as to increase the degree of bone mineralization. But there is no resulting bone over-mineralization. (incadronate) administration on the mean degree of secondary mineralization in bone.

摘要

目的

评估长期给予双膦酸盐（因卡膦酸）对继发性骨矿化平均程度的影响。

方法

30只成年比格犬根据体重随机分为三组，即CNT组、YML组和YMH组（每组5只雄性和5只雌性）。CNT组动物口服乳糖12 mg·kg⁻¹·d⁻¹，而YML组和YMH组动物分别口服因卡膦酸二钠，剂量为0.3 mg·kg⁻¹·d⁻¹和0.6 mg·kg⁻¹·d⁻¹。给药过程持续三年。在处死前，所有动物静脉注射盐酸土霉素（20 mg·kg⁻¹）进行双重标记。尸检后，切除左第9肋进行评估。

结果

组织形态计量学显示，YML组和YMH组的激活频率[（4.93±0.92）/mm²和（2.50±0.78）/mm²]每年显著低于CNT组[（7.83±0.96）/mm²]。YML组和YMH组骨矿化平均程度（1.40±0.12，1.48±0.09）显著高于CNT组（1.07±0.06）。增幅分别为31%和38%。