Institute of Engineering Thermophysics, Chongqing University, Chongqing 400030, China.
Bioresour Technol. 2010 Jun;101(11):4034-41. doi: 10.1016/j.biortech.2010.01.045.
Effects of operation parameters on hydrogen production rate, substrate utilization efficiency and hydrogen bioconversion yield were investigated in a photobioreactor packed with sodium alginate/polyvinyl alcohol-124/carrageenan granules containing Rhodopseudomonas palustris CQK 01. Results demonstrate that an increase in influent glucose concentration and flow rate enhanced glucose transport from the bulk liquid into the granules, resulting in high hydrogen production. The bacteria mainly utilized the photo-heterotrophic pathway under optimal light illumination and produced hydrogen at low influent substrate loading, while they produced organic acids at high influent loading. The optimal temperature and pH of the influent medium for hydrogen production were independent of the illumination wavelength. The maximal hydrogen production rate of 2.61mmol/L/h was achieved under illumination at 590nm and 6000lx, a substrate concentration of 60mmol/L, a temperature of 30 degrees C and a pH7 of the influent medium.
在填充有含沼泽红假单胞菌 CQK 01 的海藻酸钠/聚乙烯醇-124/卡拉胶颗粒的光生物反应器中，研究了操作参数对产氢率、基质利用效率和氢生物转化率的影响。结果表明，进水葡萄糖浓度和流速的增加促进了葡萄糖从主体液相向颗粒内的传输，从而产生了高的产氢量。在最佳光照下，细菌主要利用光异养途径，在低进水基质负荷下产氢，而在高进水负荷下产有机酸。产氢的最佳进水温度和 pH 值与光照波长无关。在 590nm 和 6000lx 光照、底物浓度为 60mmol/L、温度为 30°C、进水 pH 值为 7 的条件下，最大产氢率为 2.61mmol/L/h。
