Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, Prague, Czech Republic.
Chem Phys Lipids. 2010 May;163(4-5):373-80. doi: 10.1016/j.chemphyslip.2010.01.007. Epub 2010 Feb 4.
We used reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) for direct analysis of triacylglycerols (TAGs) from four different cultivations of Rhodococcus erythropolis CCM 2595. This technique enabled us to identify and quantify the specific molecular species of TAGs directly from lipid extracts of the bacterium, including the determination of their basic characteristics such as retention time and mass spectra. A total of 17 TAGs having at least one odd-numbered-chain FA (fatty acid) were found. This is the first time when odd-chain TAGs, predominantly with pentadecanoic, margaric, and margaroleic acids, were identified. Multiple linear regression analyses were used for predicting retention times of molecular species, predominantly of odd-chain TAGs. Cultivations on two different substrates at two different temperatures (20 and 30 degrees C) showed that only temperature had any effect on the content of TAGs. In cultivations with succinate as a carbon source the content of these TAGs increased by half (i.e., from 23.5 to 34.2%) when the cultivation temperature was lowered from 30 to 20 degrees C. The content of the PoPoMo, PoMoO and PPoMo (see Table 1) TAGs containing the odd-numbered-chain margaroleic acid rose from 0.0 to 7.1% while in cultivation on phenol as a carbon source the lowering of cultivation temperature caused an increase of these TAGs from 0.5 to 6.7%.
我们使用反相液相色谱-大气压化学电离质谱（RP-HPLC/MS-APCI）直接分析了来自四种不同培养条件下的红球菌（Rhodococcus erythropolis）CCM 2595 的三酰基甘油（TAG）。该技术使我们能够直接从细菌的脂质提取物中鉴定和定量特定的TAG 分子种类，包括确定它们的基本特征，如保留时间和质谱。总共发现了 17 种至少含有一个奇数链 FA（脂肪酸）的 TAG。这是首次鉴定出含有奇数链的 TAG，主要是十五烷酸、马尿酸和马尿酸酸。多元线性回归分析用于预测分子种类的保留时间，主要是奇数链 TAG 的保留时间。在两种不同的底物和两种不同的温度（20 和 30°C）下进行培养，结果表明只有温度对 TAG 的含量有影响。在以琥珀酸作为碳源的培养中，当培养温度从 30°C降低到 20°C 时，这些 TAG 的含量增加了一半（即从 23.5%增加到 34.2%）。含有奇数链马尿酸酸的 PoPoMo、PoMoO 和 PPoMo（见表 1）TAG 的含量从 0.0%增加到 7.1%，而在以苯酚作为碳源的培养中，降低培养温度会导致这些 TAG 的含量从 0.5%增加到 6.7%。
