U.S. Department of Agriculture, Agricultural Research Service, Bacterial Epidemiology and Antimicrobial Resistance Research Unit, Richard B. Russell Agricultural Research Center, 950 College Station Road, Athens, GA 30604-2720, United States.
J Microbiol Methods. 2010 May;81(2):96-100. doi: 10.1016/j.mimet.2010.01.024. Epub 2010 Feb 4.
Many of the Enterobacteriaceae carry multiple drug resistance (MDR) genes on large plasmids of replicon type Inc A/C and Inc H1. It is important to understand the transmission of these MDR plasmids because the genes they carry can affect the outcome of antimicrobial therapy. The aim of this study was to design a microarray with oligonucleotide probes for every gene in the six Inc A/C and one Inc H1 plasmids of interest while representing all redundant sequences only once. The microarray is printed in triplicate with 493 unique oligonucleotide probes 70 nucleotides in length. Salmonella enterica and Escherichia coli control strains and test plasmids (in the parent strain and transformed into a known E. coli background strain) were hybridized to the plasmid microarray. This hybridization arrays presents a rapid and cost effective method for high-density screening of isolates to evaluate the gene content of Inc A/C and H1 plasmids and will show how plasmids can change content with transmission.
许多肠杆菌科细菌在复制子类型 Inc A/C 和 Inc H1 的大型质粒上携带多种药物耐药 (MDR) 基因。了解这些 MDR 质粒的传播非常重要，因为它们携带的基因可能会影响抗菌治疗的结果。本研究的目的是设计一种微阵列，其中包含 6 个 Inc A/C 和 1 个 Inc H1 感兴趣质粒中每个基因的寡核苷酸探针，同时仅代表一次所有冗余序列。微阵列以三倍体形式打印，共有 493 个 70 个核苷酸长的独特寡核苷酸探针。将肠炎沙门氏菌和大肠杆菌对照菌株和测试质粒（在亲本菌株中并转化为已知的大肠杆菌背景菌株）杂交到质粒微阵列上。这种杂交阵列提供了一种快速且具有成本效益的高密度筛选方法，用于评估 Inc A/C 和 H1 质粒的基因含量，并将显示质粒如何随着传播而改变内容。
