Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China.
Mol Phylogenet Evol. 2010 Jun;55(3):1167-76. doi: 10.1016/j.ympev.2010.01.039. Epub 2010 Feb 4.
The sinipercid fish represent a group of 12 species of freshwater percoid fish endemic to East Asia. To date published morphological and molecular phylogenetics hypotheses of sinipercid fish are part congruent, and there are some areas of significant disagreement with respect to species relationships. The present study used separate and combined methods to analyze 7307 bp of data from three mitochondrial genes (cyt b, CO1 and 16S rRNA; approximately 2312 bp) and three nuclear genes (viperin, the first two introns of S7 ribosomal protein gene; approximately 4995 bp) for the attempts to estimate the relationships among sinipercids and to assess the phylogenetic utility of these markers. Phylogenetic trees were reconstructed using maximum parsimony, maximum likelihood and partitioned Bayesian analyses. Despite the detection of significant heterogeneity of phylogenetic signal between the mitochondrial and nuclear partitions, the combined data analysis represented the best-supported topology of all data. The sinipercid fish form a monophyletic group with two distinct clades, one corresponding to the genus Siniperca and the other to Coreoperca. Coreoperca whiteheadi is the sister taxon to Coreoperca herzi plus Coreoperca kawamebari. In the Siniperca, Siniperca undulata is the sister taxon to the other members of Siniperca, within the subclade containing the other members of the genus, Siniperca chuatsi and Siniperca kneri are sister species, next joined by Siniperca obscura, Siniperca roulei, Sinipercascherzeri and finally by Siniperca fortis. The potential utilities of six different genes for phylogenetic resolution of closely related sinipercid species were also evaluated, with special interest in that of the novel virus-induced protein (viperin) gene.
鱨形目鱼类代表了东亚特有的 12 种淡水鲈形目鱼类。迄今为止，已发表的鱨形目鱼类形态学和分子系统发育假说在部分上是一致的，而在种间关系方面存在一些显著分歧的领域。本研究使用单独和联合的方法分析了来自三个线粒体基因（细胞色素 b、CO1 和 16S rRNA；约 2312bp）和三个核基因（viperin、S7 核糖体蛋白基因的前两个内含子；约 4995bp）的 7307bp 数据，试图估计鱨形目鱼类之间的关系，并评估这些标记的系统发育效用。使用最大简约法、最大似然法和分区贝叶斯分析重建了系统发育树。尽管在线粒体和核分区之间检测到系统发育信号的显著异质性，但联合数据分析代表了所有数据的最佳支持拓扑结构。鱨形目鱼类形成一个单系群，有两个不同的分支，一个分支对应于 Siniperca 属，另一个分支对应于 Coreoperca。Coreoperca whiteheadi 是 Coreoperca herzi 和 Coreoperca kawamebari 的姊妹类群。在 Siniperca 中，Siniperca undulata 是 Siniperca chuatsi 和 Siniperca kneri 的姊妹类群，它们属于该属的其他成员，接下来是 Siniperca obscura、Siniperca roulei、Sinipercascherzeri，最后是 Siniperca fortis。还评估了六种不同基因对亲缘关系密切的鱨形目鱼类的系统发育分辨率的潜在效用，特别关注新型病毒诱导蛋白（viperin）基因。
