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16s rDNA 和细胞色素 b 核糖体标记在水生物种谱系和鱼类种群结构研究中的应用。

Application of 16s rDNA and cytochrome b ribosomal markers in studies of lineage and fish populations structure of aquatic species.

机构信息

Institute of Systems Biology, Universiti Kebangsaan Malaysia, UKM, 43600, Bangi, Selangor, Malaysia.

出版信息

Mol Biol Rep. 2012 May;39(5):5225-32. doi: 10.1007/s11033-011-1320-2. Epub 2011 Dec 14.

Abstract

The most economically important form of aquaculture is fish farming, which is an industry that accounts for an ever increasing share of world fishery production. Molecular markers can be used to enhance the productivity of the aquaculture and fish industries to meet the increasing demand. Molecular markers can be identified via a DNA test regardless of the developmental stage, age or environmental challenges experienced by the organism. The application of 16s and cytochrome b markers has enabled rapid progress in investigations of genetic variability and inbreeding, parentage assignments, species and strain identification and the construction of high resolution genetic linkage maps for aquaculture fisheries. In this review, the advantages of principles and potential power tools of 16s and cytochrome b markers are discussed. Main findings in term of trend, aspects and debates on the reviewed issue made from the model of aquatic species for the benefit of aquaculture genomics and aquaculture genetics research are discussed. The concepts in this review are illustrated with various research examples and results that relate theory to reality and provide a strong review of the current status of these biotechnology topics.

摘要

最具经济重要性的水产养殖形式是鱼类养殖,这是一个在世界渔业生产中所占份额不断增加的产业。分子标记可用于提高水产养殖和鱼类产业的生产力,以满足不断增长的需求。无论生物体经历何种发育阶段、年龄或环境挑战,都可以通过 DNA 测试来识别分子标记。16s 和细胞色素 b 标记物的应用使得遗传变异性和近亲繁殖、亲子关系、物种和品系鉴定以及水产养殖渔业高分辨率遗传连锁图谱的构建方面取得了快速进展。在这篇综述中,讨论了 16s 和细胞色素 b 标记物的原理和潜在强大工具的优势。根据水生物种模型讨论了该综述主题的趋势、方面和争论的主要发现,以期造福水产养殖基因组学和水产养殖遗传学研究。通过各种研究实例和结果来说明本综述中的概念,这些实例和结果将理论与实际联系起来,对这些生物技术主题的现状进行了有力的综述。

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