Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden.
Fertil Steril. 2010 May 15;93(8):2621-6. doi: 10.1016/j.fertnstert.2009.12.044.
To study the role of a synthetic insulin-like growth factor-I receptor (IGF-IR) antagonist, picropodophyllin, for mouse preimplantation embryo development in vivo and in vitro.
In vitro and in vivo study.
Hospital-based research unit.
FVB/N mice and mouse embryos.
INTERVENTION(S): The effect of picropodophyllin in mouse embryo development in vivo and in vitro, immunohistochemistry, ELISA, polymerase chain reaction.
MAIN OUTCOME MEASURE(S): Embryo development, presence of IGF-IR, messenger RNA expression, IGF-I synthesis.
RESULT(S): The effect of picropodophyllin on embryo development in vitro and in vivo was not reversible. Mice treated with picropodophyllin 1 to 3 days after mating had a reduced number of blastocysts, 40.5% versus 78.8%, and a higher number of embryos with delayed development, 48.6% versus 11.5%. Insulin-like growth factor-IR protein is present in both phosphorylated and nonphosphorylated form at all stages of embryo development. The relative IGF-IR messenger RNA expression was highest in the oocyte and reduced during development to blastocyst stage. Insulin-like growth factor-I in culture media was reduced after picropodophyllin treatment.
CONCLUSION(S): We conclude that IGF-I has an important role in normal mouse embryo development and that its receptor plays an essential role in the embryonic genome activation process.
研究合成胰岛素样生长因子-I 受体（IGF-IR）拮抗剂苦鬼白醇对体内和体外小鼠植入前胚胎发育的作用。
体内和体外研究。
医院研究单位。
FVB/N 小鼠和小鼠胚胎。
苦鬼白醇对体内和体外小鼠胚胎发育的影响，免疫组织化学，酶联免疫吸附试验，聚合酶链反应。
胚胎发育、IGF-IR 存在、信使 RNA 表达、IGF-I 合成。
苦鬼白醇对胚胎发育的影响在体内和体外均不可逆转。交配后 1 至 3 天接受苦鬼白醇治疗的小鼠囊胚数量减少，分别为 40.5%和 78.8%，发育延迟的胚胎数量更多，分别为 48.6%和 11.5%。胰岛素样生长因子-IR 蛋白在胚胎发育的各个阶段均以磷酸化和非磷酸化形式存在。卵母细胞中相对 IGF-IR 信使 RNA 表达最高，在发育到囊胚阶段时降低。苦鬼白醇处理后培养基中的胰岛素样生长因子-I 减少。
我们得出结论，IGF-I 在正常小鼠胚胎发育中具有重要作用，其受体在胚胎基因组激活过程中发挥重要作用。
