Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Química, Instituto de Investigación y Análisis Alimentario, Universidad de Santiago de Compostela, E-15782 Santiago de Compostela, Spain.
J Chromatogr A. 2010 Mar 19;1217(12):1882-90. doi: 10.1016/j.chroma.2010.01.055. Epub 2010 Jan 25.
Fragrances are ubiquitous pollutants in the environment, present in the most of household products, air fresheners, insecticides and cosmetics. Commercial perfumes may contain hundreds of individual fragrance chemicals. In addition to the widespread use and exposure to fragranced products, many of the raw fragrance materials have limited available health and safety data. Because of their nature as artificial fragrances, inhalation should be considered as an important exposure pathway, especially in indoor environments. In this work, a very simple, fast, and sensitive methodology for the analysis of 24 fragrance allergens in indoor air is presented. Considered compounds include those regulated by the EU Directive, excluding limonene; methyl eugenol was also included due to its toxicity. The proposed methodology is based on the use of a very low amount of adsorbent to retain the target compounds, and the rapid ultrasound-assisted solvent extraction (UAE) using a very low volume of solvent which avoids further extract concentration. Quantification was performed by gas chromatography coupled to mass spectrometry (GC-MS). The influence of main factors involved in the UAE step (type of adsorbent and solvent, solvent volume and extraction time) was studied using an experimental design approach to account for possible factor interactions. Using the optimized procedure, 0.2 m(-3) air are sampled, analytes are retained on 25 mg Florisil, from which they are extracted by UAE (5 min) with 2 mL ethyl acetate. Linearity was demonstrated in a wide concentration range. Efficiency of the total sampling-extraction process was studied at several concentration levels (1, 5 and 125 microg m(-3)), obtaining quantitative recoveries, and good precision (RSD<10%). Method detection limits were < or =0.6 microg m(-3). Finally, the proposed method was applied to real samples collected in indoor environments in which several of the target compounds were determined.
香料是环境中无处不在的污染物，存在于大多数家庭产品、空气清新剂、杀虫剂和化妆品中。商业香水可能含有数百种单独的香料化学品。除了广泛使用和接触香料产品外，许多原始香料材料的健康和安全数据有限。由于它们是人工香料，因此应将吸入视为重要的暴露途径，尤其是在室内环境中。在这项工作中，提出了一种非常简单、快速和灵敏的分析室内空气中 24 种香料过敏原的方法。考虑到的化合物包括欧盟指令规定的那些，不包括柠檬烯；由于其毒性，还包括甲基丁香酚。所提出的方法基于使用非常少量的吸附剂来保留目标化合物，以及使用非常少量的溶剂进行快速超声辅助溶剂萃取 (UAE)，从而避免进一步浓缩提取物。通过气相色谱-质谱联用 (GC-MS) 进行定量。使用实验设计方法研究了 UAE 步骤中涉及的主要因素（吸附剂和溶剂的类型、溶剂体积和提取时间）的影响，以考虑可能的因素相互作用。使用优化的程序，以 0.2 m(-3)的空气进行采样，将分析物保留在 25 mg Florisil 上，然后通过 UAE（5 分钟）用 2 mL 乙酸乙酯进行提取。在较宽的浓度范围内证明了线性。在几个浓度水平（1、5 和 125 μg m(-3)) 研究了整个采样-萃取过程的效率，得到了定量回收率和良好的精密度（RSD<10%）。方法检测限<或=0.6 μg m(-3)。最后，将提出的方法应用于在室内环境中收集的实际样品，其中确定了几种目标化合物。
