Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
J Plant Physiol. 2010 Jul 1;167(10):771-8. doi: 10.1016/j.jplph.2010.01.004.
The phloem is the major transport route for both small substances and large molecules, such as proteins and RNAs, from their sources to sink tissues. To investigate the proteins present in pumpkin phloem sap, proteome analysis using multidimensional protein identification technology was carried out. Pumpkin phloem peptides obtained by liquid chromatography/mass spectrometry/mass spectrometry were searched against pumpkin protein data derived from the National Center for Biotechnology Information. A total of 47 pumpkin phloem proteins were identified. The identified proteins mainly corresponded to enzymes involved in gibberellin biosynthesis, antioxidation processes, or defense mechanisms. Interestingly, seven enzymes required for gibberellin biosynthesis were identified for the first time by this proteomics approach. In summary, the new phloem proteins identified in this study provide strong evidence for stress and defense signaling and new insights regarding the role of gibberellin in the developmental programming of higher plants through the phloem.
韧皮部是小分子和大分子（如蛋白质和 RNA）从源到汇组织的主要运输途径。为了研究南瓜韧皮部汁液中的蛋白质，使用多维蛋白质鉴定技术进行了蛋白质组分析。通过液相色谱/质谱/质谱获得的南瓜韧皮肽与美国国家生物技术信息中心衍生的南瓜蛋白质数据库进行了搜索。共鉴定出 47 种南瓜韧皮部蛋白。鉴定出的蛋白质主要对应于参与赤霉素生物合成、抗氧化过程或防御机制的酶。有趣的是，通过这种蛋白质组学方法首次鉴定出赤霉素生物合成所需的七种酶。总之，本研究中鉴定的新韧皮部蛋白为应激和防御信号提供了有力证据，并为赤霉素在高等植物发育编程中的作用提供了新的见解。
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