通过位点特异性免疫制备抗9-去甘氨酰胺-8-精氨酸加压素特异性抗血清。
Development of specific antisera against 9-desglycinamide-8-arginine vasopressin by site-specific immunization.
作者信息
van Bree J B, van Nispen J W, Verhoef J C, Breimer D D
机构信息
Division of Pharmacology, Leiden University, The Netherlands.
出版信息
J Pharm Sci. 1991 Jan;80(1):46-9. doi: 10.1002/jps.2600800112.
Four specific and sensitive antisera against 9-desglycinamide-8-arginine vasopressin (DGAVP) were obtained by means of site-specific immunization in rabbits, using a Keyhole Limpet haemocyanin-DGAVP conjugate synthesized by a specific reaction of a thiol function with a maleimide group. Compared with conventionally raised antisera, the cross-reactivity with endogenous arginine vasopressin (AVP) and vasopressin-derived fragments was substantially lower. Interassay variability was 18.1% at maximum. Possible applicability of the new antisera in pharmacokinetic studies of DGAVP with respect to blood-brain barrier transport was tested by means of a pilot study in the rat.
通过在兔中进行位点特异性免疫，使用硫醇官能团与马来酰亚胺基团的特异性反应合成的钥孔戚血蓝蛋白 - 9 - 去甘氨酰胺 - 8 - 精氨酸加压素（DGAVP）缀合物，获得了四种针对DGAVP的特异性和敏感性高的抗血清。与传统制备的抗血清相比，其与内源性精氨酸加压素（AVP）和加压素衍生片段的交叉反应性显著降低。测定间的最大变异系数为18.1%。通过在大鼠中进行的一项初步研究，测试了新抗血清在DGAVP血脑屏障转运药代动力学研究中的可能适用性。
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