Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, PMB 5025, Awka, Anambra State, Nigeria.
Bioresour Technol. 2010 Jun;101(11):4120-4. doi: 10.1016/j.biortech.2009.12.057.
Two fungi characterized as Aspergillus fumigatus and Aspergillus niger, isolated from decaying cassava peels were used to convert cassava wastes by the semi-solid fermentation technique to phosphate biofertilizer. The isolates solubilized Ca(3)(PO(4))(2), AlPO(4) and FePO(4) in liquid Pikovskaya medium, a process that was accompanied by acid production. Medium for the SSF fermentation was composed of 1% raw cassava starch and 3% poultry droppings as nutrients and 96% ground (0.5-1.5mm) dried cassava peels as carrier material. During the 14days fermentation, both test organisms increased in biomass in this medium as indicated by increases in phosphatase activity and drop in pH. Ground cassava peels satisfied many properties required of carrier material particularly in respect of the organisms under study. Biofertilizer produced using A. niger significantly (p<.05) improved the growth of pigeon pea [Cajanus cajan (L.) Millsp.] in pot experiments but product made with A. fumigatus did not.
两种真菌,分别鉴定为烟曲霉和黑曲霉,从腐烂的木薯皮中分离出来,用于通过半固态发酵技术将木薯废物转化为磷酸盐生物肥料。这些分离物在液体 Pikovskaya 培养基中溶解了 Ca(3)(PO(4))(2)、AlPO(4) 和 FePO(4),这一过程伴随着产酸。半固态发酵培养基由 1%的生木薯淀粉和 3%的禽粪作为养分,96%的粉碎(0.5-1.5 毫米)干燥的木薯皮作为载体材料组成。在 14 天的发酵过程中,两种试验菌在该培养基中生物量增加,表现为磷酸酶活性增加和 pH 值下降。粉碎的木薯皮满足了载体材料所需的许多特性,特别是在研究中的这些菌方面。使用黑曲霉生产的生物肥料显著(p<.05)促进了盆栽实验中羽扇豆[Cajanus cajan (L.) Millsp.]的生长,但用烟曲霉生产的产品则没有。