Institute of Pathology, Universitätsklinikum Jena, Ziegenmühlenweg, Jena, Germany.
Pathol Res Pract. 2010 Apr 15;206(4):229-34. doi: 10.1016/j.prp.2009.11.007. Epub 2010 Feb 6.
HPV infection has been associated with head and neck carcinoma (HNSCC). In the present study, a tumor collective from Middle Germany was analyzed using two HPV-detection methods, i.e., PCR followed by agarose gel electrophoresis and microarray chip hybridization. In addition, the predictive value of tumor morphology was assessed. In total, 76 HNSCCs from 52 patients were examined, including 23 pairs of primaries and corresponding metastases. The highest rate of positive tumors was observed in the tonsils, with 76% of the patients and 81% of tumor samples being positive for high-risk HPV. DNA chip analysis detected significantly more HPV-positive cases than did agarose gel electrophoresis. Except for one HPV-33-positive case, all others tumors harbored HPV 16. There was a good concordance between the HPV status of the primary tumor and its corresponding metastasis (21/23 cases, 91%). In two cases, HPV was found only in the primary tumor but not in the metastasis. Our results clearly confirmed the high prevalence of HR-HPV in tonsillar carcinomas with a rate that was even 20% higher than those reported in the literature. Morphology is a valuable indicator for an HPV association that needs to be confirmed by molecular tests.
HPV 感染与头颈部癌（HNSCC）有关。在本研究中，使用两种 HPV 检测方法，即聚合酶链反应（PCR）后琼脂糖凝胶电泳和微阵列芯片杂交，对来自德国中部的肿瘤进行了分析。此外，还评估了肿瘤形态的预测价值。总共检查了 52 名患者的 76 例 HNSCC，包括 23 对原发灶和相应的转移灶。在扁桃体中观察到阳性肿瘤的比例最高，76%的患者和 81%的肿瘤样本呈高危 HPV 阳性。DNA 芯片分析检测到的 HPV 阳性病例明显多于琼脂糖凝胶电泳。除了一个 HPV-33 阳性病例外，所有其他肿瘤均携带 HPV16。原发肿瘤和相应转移灶的 HPV 状态之间存在良好的一致性（21/23 例，91%）。在两种情况下，HPV 仅在原发肿瘤中发现，而在转移灶中未发现。我们的结果清楚地证实了 HR-HPV 在扁桃体癌中的高流行率，其比率甚至比文献报道的高 20%。形态学是 HPV 相关性的一个有价值的指标，需要通过分子测试来证实。
