Korea University, Anam-dong, Seongbuk-gu, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2010 Mar 5;393(2):314-8. doi: 10.1016/j.bbrc.2010.01.128. Epub 2010 Feb 6.
O-linked N-acetylglucosamine (O-GlcNAc), a monosaccharide N-acetylglucosamine on the serine and threonine residues of nucleocytoplasmic proteins, is a novel protein modification that is ubiquitous among eukaryotes and implicated in cell regulation. Recent evidence indicates that O-GlcNAc regulates protein-protein interactions. Here we provide evidence that O-GlcNAc interrupts a known interaction between Sp1 and sterol regulatory element binding protein 2 (SREBP2), thereby inhibiting expression of the gene encoding acetyl-CoA synthetase 1, which is involved in lipid synthesis. This study suggests a novel mechanism in which lipid biosynthesis may be regulated by O-GlcNAc.
O-连接的 N-乙酰葡萄糖胺（O-GlcNAc）是一种在核细胞质蛋白的丝氨酸和苏氨酸残基上的单糖 N-乙酰葡萄糖胺，是一种新型的蛋白质修饰，普遍存在于真核生物中，并与细胞调节有关。最近的证据表明，O-GlcNAc 调节蛋白质-蛋白质相互作用。在这里，我们提供的证据表明，O-GlcNAc 中断了 Sp1 和固醇调节元件结合蛋白 2（SREBP2）之间的已知相互作用，从而抑制了编码乙酰辅酶 A 合成酶 1 的基因的表达，该基因参与脂质合成。这项研究提出了一种新的机制，即脂质生物合成可能受 O-GlcNAc 调控。
