Lim Kihong, Chang Hyo-Ihl
School of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.
Biochem Biophys Res Commun. 2009 Mar 13;380(3):569-74. doi: 10.1016/j.bbrc.2009.01.121. Epub 2009 Jan 25.
The novel protein modification, O-linked N-acetylglucosamine (O-GlcNAc), plays an important role in various aspects of cell regulation. Although most of nuclear transcription regulatory factors are modified by O-GlcNAc, O-GlcNAc effects on transcription remain largely undefined yet. In this study, we show that O-GlcNAc inhibits a physical interaction between Sp1 and Elf-1 transcription factors, and negatively regulates transcription of placenta and embryonic expression oncofetal protein gene (Pem). These findings suggest that O-GlcNAc inhibits Sp1-mediated gene transcription possibly by interrupting Sp1 interaction with its cooperative factor.
新型蛋白质修饰——O-连接的N-乙酰葡糖胺（O-GlcNAc）在细胞调节的各个方面发挥着重要作用。尽管大多数核转录调节因子都被O-GlcNAc修饰，但O-GlcNAc对转录的影响在很大程度上仍不明确。在本研究中，我们发现O-GlcNAc抑制Sp1和Elf-1转录因子之间的物理相互作用，并对胎盘和胚胎表达的癌胚蛋白基因（Pem）的转录产生负调控作用。这些发现表明，O-GlcNAc可能通过中断Sp1与其协同因子的相互作用来抑制Sp1介导的基因转录。
