Department of Psychological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Pharmacol Biochem Behav. 2010 Apr;95(2):258-65. doi: 10.1016/j.pbb.2010.02.001. Epub 2010 Feb 9.
The purpose of this study was to determine if aversive effects of alcohol withdrawal could be detected in mice using the place conditioning procedure and whether the GABA(A) receptor antagonist, pentylenetetrazol (PTZ), would increase the aversive effects of alcohol withdrawal and increase the probability of detecting conditioned place aversion. Subjects were alcohol-naïve mice from a specific line selectively bred for low alcohol preference (LAP1; n=91) and were assigned to three groups: alcohol withdrawal, PTZ alone, and PTZ+alcohol withdrawal. On four trials, mice received either a 4.0 g/kg intraperitoneal (i.p.) injection of alcohol (alcohol withdrawal, PTZ+alcohol withdrawal groups) or saline (PTZ group) 8 h prior to being placed on a distinctive floor texture for a 30-min conditioning session. Five minutes before these sessions, mice in the PTZ and PTZ+alcohol withdrawal groups received PTZ (5.0 mg/kg; i.p.) and the alcohol withdrawal group received saline. On intervening days mice received two saline injections at the same time points prior to being placed on a different floor texture. Post-conditioning floor preference was assessed in two 60-min tests; the first test was drug-free and the second test was state-dependent. Neither alcohol withdrawal nor PTZ produced significant place conditioning. The PTZ+alcohol withdrawal group showed a significant place aversion during the state-dependent test. These data suggest that the combined stimulus properties of PTZ and alcohol withdrawal facilitated the expression of conditioned place aversion to alcohol withdrawal.
这项研究的目的是确定是否可以使用位置条件反射程序在小鼠中检测到酒精戒断的厌恶效应，以及 GABA(A) 受体拮抗剂戊四氮（PTZ）是否会增加酒精戒断的厌恶效应，并增加检测到条件性位置厌恶的可能性。研究对象是来自特定系的酒精-naive 小鼠，该系专门通过选择繁殖来降低对酒精的偏好（LAP1；n=91），并被分为三组：酒精戒断组、PTZ 单独组和 PTZ+酒精戒断组。在四轮试验中，小鼠接受了 4.0 g/kg 的腹腔（i.p.）酒精注射（酒精戒断、PTZ+酒精戒断组）或生理盐水（PTZ 组），然后将其置于独特的地板纹理上进行 30 分钟的条件反射训练。在这些训练前 5 分钟，PTZ 和 PTZ+酒精戒断组的小鼠接受了 PTZ（5.0 mg/kg；i.p.），而酒精戒断组则接受了生理盐水。在其余的日子里，小鼠在相同的时间点接受了两次生理盐水注射，然后被放置在不同的地板纹理上。在两次 60 分钟的测试中评估了条件反射后的地板偏好，第一次测试是无药物的，第二次测试是状态依赖性的。酒精戒断和 PTZ 都没有产生显著的位置条件反射。PTZ+酒精戒断组在状态依赖性测试中表现出明显的位置厌恶。这些数据表明，PTZ 和酒精戒断的联合刺激特性促进了对酒精戒断的条件性位置厌恶的表达。
