Cunningham Christopher L, Gremel Christina M, Groblewski Peter A
Department of Behavioral Neuroscience and Portland Alcohol Research Center, Oregon Health & Science University, Portland, Oregon 97239-3098, USA.
Nat Protoc. 2006;1(4):1662-70. doi: 10.1038/nprot.2006.279.
This protocol describes the equipment and methods used to establish conditioned place preference (CPP) or aversion (CPA). Place conditioning is a form of Pavlovian conditioning routinely used to measure the rewarding or aversive motivational effects of objects or experiences (e.g., abused drugs). Here, we present a place conditioning procedure that has been used extensively to study the motivational effects of ethanol and other abused drugs in mice. This protocol involves three phases: (i) habituation (or a pretest), (ii) conditioning of an association between the drug and a tactile or visual stimulus and (iii) a test that offers a choice between the drug-associated cue and a neutral cue. If the drug has motivational significance, mice will spend significantly more time (CPP) or less time (CPA) in proximity to the drug-associated cue. Potential problems in the design and interpretation of place conditioning studies are discussed. A typical experiment lasts 2 weeks.
本方案描述了用于建立条件性位置偏爱（CPP）或厌恶（CPA）的设备和方法。位置条件反射是一种经典的条件反射形式，常用于测量物体或经历（如滥用药物）的奖赏或厌恶动机效应。在此，我们介绍一种位置条件反射程序，该程序已被广泛用于研究乙醇和其他滥用药物对小鼠的动机效应。本方案包括三个阶段：（i）习惯化（或预测试），（ii）药物与触觉或视觉刺激之间关联的条件反射，以及（iii）在药物相关线索和中性线索之间进行选择的测试。如果药物具有动机意义，小鼠将在靠近药物相关线索的地方花费显著更多的时间（CPP）或更少的时间（CPA）。文中讨论了位置条件反射研究设计和解释中的潜在问题。一个典型的实验持续2周。
