Department of Toxicology, Anhui Medical University, Hefei 230032, China.
Food Chem Toxicol. 2010 May;48(5):1160-9. doi: 10.1016/j.fct.2010.02.003. Epub 2010 Feb 6.
Fenvalerate, a widely used pyrethroid insecticide, has been associated with poor semen quality. As yet, little is known about the effects of prenatal fenvalerate exposure on testicular development. The present study investigated the effects of prenatal fenvalerate exposure on testicular development and spermatogenesis. The pregnant mice were administered fenvalerate (30 mg/kg) by gavage daily from gestational day (gd) 13 to gd 18. The weights of testes and epididymides were significantly decreased in mice whose mothers were exposed to fenvalerate during pregnancy. Importantly, maternal fenvalerate exposure during pregnancy markedly decreased the number of mature seminiferous tubules (stages VII and VIII) in testes of adult male offspring. In addition, maternal fenvalerate exposure during pregnancy significantly reduced the number of epididymal spermatozoa in adult male offspring. Additional experiments showed that the level of serum testosterone (T) was significantly decreased in male fetuses whose mothers were exposed to fenvalerate during pregnancy. Correspondingly, mRNA and protein levels of P450(17alpha), a T synthetic enzyme, were significantly decreased in fetal testes. Moreover, the disruptive effect of prenatal fenvalerate exposure on testicular T synthesis was irreversible. In conclusion, prenatal fenvalerate exposure irreversibly impairs testicular development and spermatogenesis at least into early adulthood.
氰戊菊酯，一种广泛使用的拟除虫菊酯类杀虫剂，与精液质量差有关。然而，目前对于产前接触氰戊菊酯对睾丸发育的影响知之甚少。本研究探讨了产前接触氰戊菊酯对睾丸发育和精子发生的影响。妊娠小鼠从妊娠第 13 天（gd）到第 18 天（gd）每天通过灌胃给予氰戊菊酯（30mg/kg）。暴露于氰戊菊酯的孕鼠的睾丸和附睾重量明显降低。重要的是，母体在妊娠期间接触氰戊菊酯可明显减少成年雄性后代睾丸中成熟精小管（VII 和 VIII 期）的数量。此外，母体在妊娠期间接触氰戊菊酯可显著减少成年雄性后代附睾中的精子数量。进一步的实验表明，母体在妊娠期间接触氰戊菊酯的雄性胎儿血清睾酮（T）水平显著降低。相应地，胎儿睾丸中 T 合成酶 P450（17alpha）的 mRNA 和蛋白水平也显著降低。此外，产前接触氰戊菊酯对睾丸 T 合成的破坏作用是不可逆转的。总之，产前接触氰戊菊酯可不可逆地损害睾丸发育和精子发生，至少到成年早期。
