Department of Toxicology, Anhui Medical University, Hefei, 230032, China.
J Appl Toxicol. 2010 May;30(4):369-77. doi: 10.1002/jat.1507.
Fenvalerate, a pyrethroid insecticide used worldwide, has been shown to have a potentially adverse effect on male reproduction. Our earlier study showed that maternal fenvalerate exposure during lactation impaired testicular development in male offspring. In this study, we investigated the effects of pubertal and early adult exposure to fenvalerate on steroidogenesis and spermatogenesis in mice. Male mice were administered fenvalerate (60 mg/kg) by gavage daily from postnatal day 35 (PND35) to PND63. Results showed that sperm count was significantly decreased in fenvalerate-treated mice. In addition, fenvalerate markedly decreased the layers of spermatogenic cells, disturbed the array of spermatogenic cells and increased the number of apoptotic cells in testes. The adverse effects of fenvalerate on male reproduction seemed to be associated with a decrease in serum and testicular testosterone (T). Although pubertal and early adult exposure to fenvalerate had little effect on the number of Leydig cells in testes, mRNA and protein levels of testicular T biosynthetic enzymes including P450(17alpha) and P450scc were significantly downregulated in fenvalerate-treated mice. In conclusion, pubertal and early adult fenvalerate exposure induces a deleterious effect on steroidogenesis and spermatogenesis in adulthood. The decreased testicular T synthesis partially contributes to fenvalerate-induced impairment on spermatogenesis.
氰戊菊酯是一种在世界范围内使用的拟除虫菊酯类杀虫剂，已被证明对男性生殖有潜在的不良影响。我们之前的研究表明，哺乳期母体接触氰戊菊酯会损害雄性后代的睾丸发育。在这项研究中，我们研究了青春期和成年早期暴露于氰戊菊酯对小鼠类固醇生成和精子发生的影响。雄性小鼠从出生后第 35 天（PND35）到第 63 天（PND63）每天通过灌胃给予氰戊菊酯（60mg/kg）。结果表明，氰戊菊酯处理组的精子计数显著减少。此外，氰戊菊酯明显减少了生精细胞的层数，扰乱了生精细胞的排列，并增加了睾丸中的凋亡细胞数量。氰戊菊酯对男性生殖的不良影响似乎与血清和睾丸睾酮（T）减少有关。尽管青春期和成年早期暴露于氰戊菊酯对睾丸间质细胞的数量几乎没有影响，但氰戊菊酯处理组的睾丸 T 生物合成酶包括 P450(17alpha)和 P450scc 的 mRNA 和蛋白水平显著下调。总之，青春期和成年早期暴露于氰戊菊酯会导致成年后类固醇生成和精子发生的有害影响。睾丸 T 合成减少部分导致氰戊菊酯引起的精子发生障碍。
