通过基于隐马尔可夫模型的轮廓分析鉴定新型L-氨基酸α-连接酶。
Identification of novel L-amino acid alpha-ligases through Hidden Markov Model-based profile analysis.
作者信息
Senoo Akihiro, Tabata Kazuhiko, Yonetani Yoshiyuki, Yagasaki Makoto
机构信息
Bioprocess Development Center, Kyowa Hakko Bio Co., Ltd., Tsukuba, Ibaraki, Japan.
出版信息
Biosci Biotechnol Biochem. 2010;74(2):415-8. doi: 10.1271/bbb.90644. Epub 2010 Feb 7.
L-Amino acid alpha-ligase (Lal), catalyzing the formation of alpha-dipeptides from unprotected L-amino acids in an ATP-dependent manner, is used in cost-effective fermentative production of dipeptides. We searched for novel Lals by in silico screening using Hidden Markov Model-based profile analysis, and identified five novel Lals that showed low similarity and different substrate specificity from known Lals.
L-氨基酸α-连接酶（Lal）以ATP依赖的方式催化由未保护的L-氨基酸形成α-二肽，被用于二肽的经济高效发酵生产。我们通过基于隐马尔可夫模型的轮廓分析进行计算机筛选来寻找新型Lal，并鉴定出了五种新型Lal，它们与已知的Lal显示出低相似性和不同的底物特异性。
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