嗜热古菌火球菌OT3中核糖核酸酶P RNA与蛋白质相互作用时诱导产生的结构变化。
Structural changes in ribonuclease P RNA in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 induced on interaction with proteins.
作者信息
Kosaka Shunsuke, Hada Kazumasa, Nakashima Takashi, Kimura Makoto
机构信息
Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.
出版信息
Biosci Biotechnol Biochem. 2010;74(2):394-6. doi: 10.1271/bbb.90577. Epub 2010 Feb 7.
The activated structure of RNase P RNA (PhopRNA) in Pyrococcus horikoshii OT3 was characterized by circular dichroism (CD) and ultraviolet (UV) absorbance spectra. The results suggested that interaction of four RNase P proteins (PhoPop5, PhoRpp21, PhoRpp29, and PhoRpp30) with PhopRNA results in destabilization of base stacking in PhopRNA, whereas the addition of a fifth protein, PhoRpp38, increases base stacking in PhopRNA.
通过圆二色性（CD）和紫外（UV）吸收光谱对嗜热栖热菌OT3中核糖核酸酶P RNA（PhopRNA）的活化结构进行了表征。结果表明，四种核糖核酸酶P蛋白（PhoPop5、PhoRpp21、PhoRpp29和PhoRpp30）与PhopRNA的相互作用导致PhopRNA中碱基堆积的不稳定，而添加第五种蛋白PhoRpp38则增加了PhopRNA中的碱基堆积。
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