Choudhury Swarup Roy, Roy Sujit, Singh Sanjay Kumar, Sengupta Dibyendu N
Department of Botany, Bose Institute, Kolkata, West Bengal, India.
Department of Chemistry, Bose Institute, Kolkata, West Bengal, India.
Plant Signal Behav. 2010 May;5(5):553-7. doi: 10.4161/psb.11092. Epub 2010 Feb 8.
Recently, we have reported the characterization of promoter region of Sucrose phosphate synthase (SPS) gene in banana and investigated the role of some cis-elements/motifs, present in the promoter of SPS, in the transcriptional regulation of the gene. DNA-protein interaction studies have demonstrated the presence of specific trans-acting factors which showed specific interactions with ethylene, auxin, low temperature and light responsive elements in regulating SPS transcription. Transient expression analyses have demonstrated the functional significance of the various cis-acting regulatory elements present in banana SPS promoter in regulating SPS expression during ripening. (1) Here, we have further discussed the possible role of these regulatory sequences in the regulation of transcriptional network and comment on their function in relation to sucrose metabolism during banana fruit ripening.
最近，我们报道了香蕉中蔗糖磷酸合酶（SPS）基因启动子区域的特征，并研究了SPS启动子中存在的一些顺式元件/基序在该基因转录调控中的作用。DNA-蛋白质相互作用研究表明存在特定的反式作用因子，这些因子在调节SPS转录过程中与乙烯、生长素、低温和光响应元件表现出特异性相互作用。瞬时表达分析证明了香蕉SPS启动子中存在的各种顺式作用调控元件在果实成熟过程中调节SPS表达的功能意义。（1）在此，我们进一步讨论了这些调控序列在转录网络调控中的可能作用，并对它们在香蕉果实成熟期间与蔗糖代谢相关的功能进行了评论。
