Hanna P C, McClane B A
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Mol Microbiol. 1991 Jan;5(1):225-30. doi: 10.1111/j.1365-2958.1991.tb01843.x.
Clostridium perfringens enterotoxin (CPE) is believed to be involved in several important gastrointestinal illnesses. Recent studies have identified a number of distinct molecular events which occur after CPE treatment of eukaryotic cells or isolated membranes. Additional studies are underway to determine the temporal order and intrinsic importance of each CPE event for cytotoxicity. We now demonstrate that a truncated CPE fragment binds to membranes, but is unable to insert into membranes or cause any other subsequent post-insertion event. This is the first experimental evidence supporting the importance of membrane insertion for CPE cytotoxicity. Binding of the CPE fragment is also shown to be irreversible, strongly suggesting that the irreversible binding of wild-type CPE is not due solely to insertion of CPE into membranes.
产气荚膜梭菌肠毒素（CPE）被认为与多种重要的胃肠道疾病有关。最近的研究已经确定了一些在CPE处理真核细胞或分离的膜后发生的不同分子事件。正在进行进一步的研究，以确定每个CPE事件对细胞毒性的时间顺序和内在重要性。我们现在证明，一个截短的CPE片段能与膜结合，但不能插入膜中或引起任何其他随后的插入后事件。这是支持膜插入对CPE细胞毒性重要性的首个实验证据。CPE片段的结合也被证明是不可逆的，这有力地表明野生型CPE的不可逆结合并非仅仅归因于CPE插入膜中。
