Max Planck Institute for Plant Breeding Research, Carl von Linné Weg 10, Köln, Germany.
Genome. 2010 Feb;53(2):103-10. doi: 10.1139/g09-086.
Potato chromosome 5 harbours numerous genes for important qualitative and quantitative traits, such as resistance to the root cyst nematode Globodera pallida and the late blight fungus, Phytophthora infestans. The genes make up part of a "hot spot" for resistances to various pathogens covering a genetic map length of 3 cM between markers GP21 and GP179. We established the physical size and position of this region on chromosome 5 in potato and tomato using fluorescence in situ hybridization (FISH) on pachytene chromosomes. Five potato bacterial artificial chromosome (BAC) clones with the genetically anchored markers GP21, R1-contig (proximal end), CosA, GP179, and StPto were selected, labeled with different fluorophores, and hybridized in a five-colour FISH experiment. Our results showed the location of the BAC clones in the middle of the long arm of chromosome 5 in both potato and tomato. Based on chromosome measurements, we estimate the physical size of the GP21-GP179 interval at 0.85 Mb and 1.2 Mb in potato and tomato, respectively. The GP21-GP179 interval is part of a genome segment known to have inverted map positions between potato and tomato.
马铃薯 5 号染色体上有许多重要的定性和定量性状的基因，如对根结线虫 Globodera pallida 和晚疫病病原菌 Phytophthora infestans 的抗性。这些基因构成了一个“热点”的一部分，该“热点”包含了在标记 GP21 和 GP179 之间遗传图谱长度为 3 cM 的各种病原体抗性的基因。我们利用粗线期染色体的荧光原位杂交（FISH）技术，在马铃薯和番茄中建立了该 5 号染色体区域的物理大小和位置。选择了 5 个带有遗传锚定标记 GP21、R1 片段（近端）、CosA、GP179 和 StPto 的马铃薯细菌人工染色体（BAC）克隆，用不同的荧光染料标记，并在五色 FISH 实验中进行杂交。我们的结果表明，在马铃薯和番茄中，BAC 克隆位于 5 号染色体长臂的中间。根据染色体测量，我们估计 GP21-GP179 区间在马铃薯和番茄中的物理大小分别为 0.85 Mb 和 1.2 Mb。GP21-GP179 区间是已知在马铃薯和番茄之间存在图谱位置倒置的基因组片段的一部分。
