Borromei Chiara, Careri Maria, Cavazza Antonella, Corradini Claudio, Elviri Lisa, Mangia Alessandro, Merusi Cristiana
Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università di Parma, Viale Usberti 17/a, 43100 Parma, Italy.
Int J Anal Chem. 2009;2009:530639. doi: 10.1155/2009/530639. Epub 2009 Mar 18.
This paper describes the complementarity of high-performance anion exchange chromatography coupled with pulsed electrochemical detection (HPAEC-PED) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) to evaluate commercial available fructans (fructooligosaccharides (FOS) and inulins), having different degrees of polymerization (DP) which are usually employed by food industry as functional ingredients either for their prebiotic properties or as a fat replacer, giving a fat-like mouth feel and texture. The developed HPAEC-PED methods are able to analyze FOS (fructans with DP 3-10) and inulins (DP ranging from 3 to 80) with a good resolution and relatively short retention times to evaluate structural differences between fructooligosaccharide and inulins and the possible presence of inulooligosaccharides as well as of branching. To characterize FOS and inulin at different degrees of polymerization and to assure correct molecular assignment, MALDI-TOF MS analysis was also investigated. The 2,5-dihydroxy benzoic acid (2,5-DHB) was found to be the best matrix for FOS analysis as Actilight and Raftilose P95 products, while 3-aminoquinoline (3-AQ) seems to be the best matrix for inulin with higher DP. The applicability of the optimized methods to the identification and determination of FOS contained in a symbiotic milk as well as a type of inulin added as functional ingredient to a cooked ham is demonstrated.
本文描述了高效阴离子交换色谱-脉冲电化学检测法（HPAEC-PED）与基质辅助激光解吸/电离质谱法（MALDI-TOF-MS）的互补性，用于评估市售的果聚糖（低聚果糖（FOS）和菊粉），它们具有不同的聚合度（DP），食品工业通常将其作为功能性成分，因其具有益生元特性或作为脂肪替代品，能赋予类似脂肪的口感和质地。所开发的HPAEC-PED方法能够以良好的分辨率和相对较短的保留时间分析FOS（聚合度为3-10的果聚糖）和菊粉（聚合度范围为3至80），以评估低聚果糖和菊粉之间的结构差异以及可能存在的低聚菊糖和分支情况。为了表征不同聚合度的FOS和菊粉并确保正确的分子归属，还研究了MALDI-TOF MS分析。发现2,5-二羟基苯甲酸（2,5-DHB）是分析Actilight和Raftilose P95产品等FOS的最佳基质，而3-氨基喹啉（3-AQ）似乎是分析较高聚合度菊粉的最佳基质。证明了优化方法在鉴定和测定共生牛奶中所含FOS以及作为功能性成分添加到熟火腿中的一种菊粉的适用性。
