Lopes Sheila M S, Krausová Gabriela, Rada Vojtěch, Gonçalves José E, Gonçalves Regina A C, de Oliveira Arildo J B
Department of Pharmacy, Pharmaceutical Sciences Post-Graduate Program, State University of Maringá, Ave. Colombo 5790, 87.020-900, Maringá, Brazil.
Department of Microbiology and Technology, Dairy Research Institute, Ke Dvoru 12a, 160 00 Prague, Czech Republic.
Carbohydr Res. 2015 Jun 26;411:15-21. doi: 10.1016/j.carres.2015.03.018. Epub 2015 Apr 11.
The polysaccharide inulin has great importance in the food and pharmaceutical industries. The degree of polymerization (DP) of inulin influences important properties, such as, solubility, thermal stability, sweetness power and prebiotic activity. Molecules with a high degree of polymerization are obtained through physical techniques for enrichment of the inulin chains because they are not commonly obtained from plants extract. Gas chromatography/Mass Spectrometry and (1)H Nuclear Magnetic Resonance analysis showed that inulin from Stevia rebaudiana roots has a degree of polymerization (DPn 28) higher than the value of DPn 12-15 for inulins from other plant species. Furthermore, the methodology of freeze/thaw to enrich the chains allowed us to increase the DP, similarly to other methodologies used for the enrichment of inulin chains. The prebiotic assays confirm that inulin from S. rebaudiana has a high DP. The combined use of these molecules with low degree of polymerization fructans seems to be advantageous to prolong the prebiotic effect in the colon. Our results suggest that S. rebaudiana roots are a promising source of high degree polymerization inulins.
多糖菊粉在食品和制药行业具有重要意义。菊粉的聚合度（DP）会影响其重要特性，如溶解度、热稳定性、甜度和益生元活性。由于通常无法从植物提取物中获得高度聚合的分子，因此需通过物理技术来富集菊粉链以获取此类分子。气相色谱/质谱分析和¹H核磁共振分析表明，甜叶菊根中的菊粉聚合度（DPn 28）高于其他植物物种菊粉的DPn 12 - 15值。此外，与用于富集菊粉链的其他方法类似，冷冻/解冻富集链的方法使我们能够提高DP。益生元试验证实，甜叶菊中的菊粉具有较高的DP。将这些低聚合度果聚糖分子联合使用似乎有利于延长结肠中的益生元效应。我们的结果表明，甜叶菊根是高聚合度菊粉的一个有前景的来源。
