Research National Center of Cassava and Tropical Fruits, Brazilian Company of Agricultural Research, Cruz das Almas, BA, Brazil.
Biol Res. 2009;42(4):427-36. Epub 2010 Jan 29.
The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy. The H. alvei 071 hall mutant was deficient in biofilm formation. All these results showed that the quorum sensing system is probably involved in the regulation of biofilm formation by H. alvei.
本研究的目的是检测哈夫尼亚菌（Hafnia alvei）生物膜中群体感应系统信号分子酰基高丝氨酸内酯（AHLs）的存在，并评估人工合成的群体感应抑制剂对生物膜形成的影响。采用孔扩散技术、薄层层析（TLC）和生物监测物直接检测生物膜中的方法检测 AHLs。从浮游细胞和定殖细胞中提取的哈夫尼亚菌提取物至少诱导了三种监测菌株中的两种。通过 TLC 证实了具有多达六个碳原子的 AHLs 的存在。呋喃酮可抑制哈夫尼亚菌的生物膜形成，通过微孔板结晶紫 96 孔测定和扫描电子显微镜观察证实了这一点。哈夫尼亚菌 071 突变体在生物膜形成方面存在缺陷。所有这些结果表明，群体感应系统可能参与了哈夫尼亚菌生物膜形成的调控。
