Shen Yue, Cui Fangchao, Wang Dangfeng, Li Tingting, Li Jianrong
National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou 121013, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214000, China.
Foods. 2021 Nov 5;10(11):2700. doi: 10.3390/foods10112700.
Quorum sensing (QS) is widely present in microorganisms in marine aquatic products. Owing to the use of antibiotics, many spoilage bacteria in aquatic products are drug resistant. In order to slow down this evolutionary trend, the inhibition of spoilage phenotype of spoilage bacteria by interfering with QS has become a research hot spot in recent years. In this study, we found a new QS quenching enzyme, PF-1240; it was cloned and expressed in 08. Sequence alignment showed that its similarity with -homoserine lactone (AHL) acylase QuiP protein of ( 0-1) was 78.4%. SDS-PAGE confirmed that the protein is a dimer composed of two subunits, which is similar to the structure of AHL acylases. The concentration of heterologous expression in (DE3) was 26.64 μg/mL. Unlike most AHL acylases, PF-1240 can quench AHLs with different carbon chain lengths and inhibit the quorum sensing of the aquatic spoilage bacterium . It can significantly reduce the formation rate of biofilm of to 44.4% and the yield of siderophores to 54%, inhibit the production of protease and lipase, and interfere with the motility of . Through these corruption phenotypes, the specific application effect of PF-1240 can be further determined to provide a theoretical basis for its application in the preservation of practical aquatic products.
群体感应（QS）广泛存在于海洋水产品中的微生物中。由于抗生素的使用，水产品中的许多腐败细菌具有耐药性。为了减缓这种进化趋势，通过干扰群体感应来抑制腐败细菌的腐败表型已成为近年来的研究热点。在本研究中，我们发现了一种新的群体感应淬灭酶PF-1240；它在08中被克隆并表达。序列比对表明，它与（0-1）的高丝氨酸内酯（AHL）酰基转移酶QuiP蛋白的相似性为78.4%。SDS-PAGE证实该蛋白是由两个亚基组成的二聚体，这与AHL酰基转移酶的结构相似。在（DE3）中的异源表达浓度为26.64μg/mL。与大多数AHL酰基转移酶不同，PF-1240可以淬灭不同碳链长度的AHL，并抑制水产腐败菌的群体感应。它可以显著将的生物膜形成率降低到44.4%，铁载体产量降低到54%，抑制蛋白酶和脂肪酶的产生，并干扰的运动性。通过这些腐败表型，可以进一步确定PF-1240的具体应用效果，为其在实际水产品保鲜中的应用提供理论依据。
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