进一步评估改良 PCR 法检测人粪便样本中曼氏血吸虫 DNA。
Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples.
机构信息
Laboratório de Pesquisas Clínicas, Instituto de Pesquisas René Rachou-Fiocruz, Av Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2009 Dec;104(8):1194-6. doi: 10.1590/s0074-02762009000800021.
A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings.
先前报道的一种用于检测曼氏血吸虫 DNA 的灵敏 PCR 检测方法已得到更新和评估。DNA 提取方法的改变，包括使用全球可用的商业试剂盒和增加额外的质量控制措施，提高了检测的稳健性，对巴西一个流行地区的 67 份粪便样本的分析结果证实了这一点。该 PCR 检测方法是一种经过验证的可靠诊断检测方法，可用于特定环境中的血吸虫病控制。
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