Institute of Physiology, University of Regensburg, Universitätsstr. 31, 93040 Regensburg, Germany.
Pflugers Arch. 2010 Mar;459(4):557-67. doi: 10.1007/s00424-009-0770-z. Epub 2010 Feb 6.
Genetically modified mice, created by random integration of a transgene into the genome or by targeted mutation of a specific gene, have proven to be extremely powerful tools for studying gene function in vivo. In this article, we give (1) a short overview of the traditional methods in mouse transgenesis and (2) a discussion of the problems with these methods, (3) more recent methods that were developed to overcome these problems, and (4) an outlook on future directions in gene targeting.
遗传修饰小鼠，通过随机整合转基因或靶向特定基因的突变而产生，已被证明是研究体内基因功能的极其强大的工具。在本文中，我们（1）简要概述传统的小鼠转基因方法，（2）讨论这些方法存在的问题，（3）介绍为克服这些问题而开发的较新方法，以及（4）展望基因靶向的未来方向。
