Department of Gynecology, Suleyman Demirel University, Isparta, Turkey.
J Membr Biol. 2010 Feb;233(1-3):135-42. doi: 10.1007/s00232-010-9233-x. Epub 2010 Feb 7.
We investigated the effects of melatonin administration on ovariectomy-induced oxidative toxicity and N-methyl-D: -aspartate receptor (NMDAR) subunits in the blood of rats. Thirty-two rats were studied in three groups. The first and second groups were control and ovariectomized rats. Melatonin was daily administrated to the ovariectomized rats in the third group for 30 days. Blood, brain cortical and hippocampal samples were taken from the three groups after 30 days. Brain cortical, erythrocyte and plasma lipid peroxidation (LP) levels were higher in the ovariectomized group than in controls, although the LP level was decreased in the ovariectomized group with melatonin treatment. Brain cortical and plasma concentrations of vitamins A, C and E as well as the NMDAR 2B subunit were lower in the ovariectomized group than in controls, although, except for plasma vitamin C, they were increased by the treatment. Brain cortical and erythrocyte reduced glutathione (GSH) levels were lower in the ovariectomized group than in controls, although erythrocyte GSH levels were higher in the melatonin group than in the ovariectomized group. Brain cortical and erythrocyte glutathione peroxidase activity and NMDAR 2A subunit concentrations were not found to be different in all groups statistically. Oxidative stress has been proposed to explain the biological side effect of experimental menopause. Melatonin prevents experimental menopause-induced oxidative stress to strengthen antioxidant vitamin and NMDAR 2A subunit concentrations in ovariectomized rats.
我们研究了褪黑素给药对卵巢切除诱导的大鼠血液氧化毒性和 N-甲基-D：-天冬氨酸受体（NMDAR）亚单位的影响。 32 只大鼠分为三组进行研究。第一组和第二组为对照组和卵巢切除组。第三组的卵巢切除大鼠每天给予褪黑素治疗 30 天。30 天后，从三组中取出血液、大脑皮质和海马样本。与对照组相比，卵巢切除组的大脑皮质、红细胞和血浆脂质过氧化（LP）水平更高，但褪黑素治疗组的 LP 水平降低。与对照组相比，卵巢切除组的大脑皮质和血浆维生素 A、C 和 E 以及 NMDAR 2B 亚基浓度较低，但除了血浆维生素 C 外，这些浓度都因治疗而增加。与对照组相比，卵巢切除组的大脑皮质和红细胞还原型谷胱甘肽（GSH）水平较低，但褪黑素组的红细胞 GSH 水平高于卵巢切除组。大脑皮质和红细胞谷胱甘肽过氧化物酶活性和 NMDAR 2A 亚基浓度在所有组中均无统计学差异。氧化应激被认为可以解释实验性绝经的生物学副作用。褪黑素可预防实验性绝经引起的氧化应激，增强抗氧化维生素和 NMDAR 2A 亚基在卵巢切除大鼠中的浓度。
