Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China.
Mol Biol Rep. 2011 Jun;38(5):3153-60. doi: 10.1007/s11033-010-9986-4. Epub 2010 Feb 7.
Transgenically expressed Syndecan-1 was found in the hypothalamic nuclei that control energy balance, and was associated with maturity-onset obesity, while ghrelin has been shown to play important roles in the control of food intake, gastric acid secretion, energy homeostasis, and glucose and lipid metabolism. However, the roles of genetic variations of Syndecan-1 and ghrelin on growth trait have few been reported in cattle. Herein, five Chinese cattle breeds were analyzed by PCR-SSCP and DNA sequencing methods. The bovine ghrelin gene showed eleven SNPs g.[267G>A, 271G>A, 290C>T, 326A>G, 327T>C, 420C>A, 569A>G, 945C>T, 993C>T, 4491A>G, 4644G>A] and three SNPs g.[420C>A, 569 A>G, 945C>T] were firstly detected in cattle. The bovine Syndecan-1 gene showed two SNPs. One SNP showed a transition C>G at position 21514, resulting in a synonymous mutation p.G(GGC)169G(GGG) and another showed a transversion C>T at position 22591, resulting in a synonymous mutation p.D(GAC)283D(GAT). In ghrelin gene, no significant associations were revealed between any variant sites and body weight, average daily gain, body sizes for different growth periods (6, 12, 18, and 24 months old), as well as for the milk yield at 305 days, milk protein rate and milk fat percentage. However, the polymorphism of Syndecan-1 gene was significantly associated with bovine birth weight and body length. Hence, we first suggested that Syndecan-1 gene could be regarded as molecular marker for superior birth weight and body length.
转染表达的 Syndecan-1 存在于控制能量平衡的下丘脑核中，与成年肥胖有关，而 ghrelin 已被证明在控制食物摄入、胃酸分泌、能量平衡以及葡萄糖和脂质代谢方面发挥着重要作用。然而， Syndecan-1 和 ghrelin 的遗传变异对生长性状的影响在牛中鲜有报道。本研究采用 PCR-SSCP 和 DNA 测序方法对 5 个中国牛品种进行了分析。牛 ghrelin 基因显示 11 个 SNPs g.[267G>A、271G>A、290C>T、326A>G、327T>C、420C>A、569A>G、945C>T、993C>T、4491A>G、4644G>A]和 3 个 SNPs g.[420C>A、569A>G、945C>T]首次在牛中检测到。牛 Syndecan-1 基因显示 2 个 SNPs。一个 SNP 在位置 21514 处发生转换 C>G，导致同义突变 p.G(GGC)169G(GGG)，另一个 SNP 在位置 22591 处发生颠换 C>T，导致同义突变 p.D(GAC)283D(GAT)。在 ghrelin 基因中，没有发现任何变异位点与体重、平均日增重、不同生长时期（6、12、18 和 24 月龄）以及 305 天产奶量、乳蛋白率和乳脂率之间存在显著相关性。然而， Syndecan-1 基因的多态性与牛的初生重和体尺显著相关。因此，我们首次提出 Syndecan-1 基因可作为牛优良初生重和体尺的分子标记。
