Institute of Cellular and Molecular Biology, Xuzhou Normal University, No. 101 Shanghai Road, 221116 Xuzhou, Jiangsu, People's Republic of China.
Mol Biol Rep. 2011 Jun;38(5):3129-35. doi: 10.1007/s11033-010-9983-7. Epub 2010 Feb 7.
Somatostatin (SST) and its receptors (SSTR1-5) appear to be important in central regulation of many metabolic systems that affect growth, adiposity and nutrient absorption. In this study, we investigated polymorphisms within the caprine SST and SSTR1 genes and determined their relationship with growth traits. As there were no sequence information of the caprine SST and SSTR1 genes, we explored their DNA sequence and genomic organizations. The caprine SST gene is organized in two exons and is transcribed into an mRNA containing 351 bp of sequence coding for a protein of 116 amino acids. Its protein sequences showed substantial similarity (97-99%) to its respective orthologs from cattle, human and mouse. We also cloned and sequenced a 1.2 kb DNA fragment which contained the major part of the coding region and 3' UTR of the caprine SSTR1 gene. We then detected the polymorphisms in these determined sequences by PCR-SSCP and DNA sequencing methods in 459 goats from four breeds. Four SNPs (GU014693:g.647T>C, GU014693:g.844A>C, GU014693:g.970T>C, GU014693:g.1039T>A), segregating as two haplotypes (T-A-T-T and C-C-C-A), were identified in intron 1 of the caprine SST gene and showed the associations to body length and body height (P < 0.05). Two SNPs (GU014695:g.801 C>T, GU014695:g.948 C>T) were identified in the caprine SSTR1 gene. Significant associations between the three genotypes of GU014695:801 C>T and body length, body height, and chest circumference was observed (P < 0.05). These results suggest that the caprine SST and SSTR1 genes are strong candidate genes that influence growth traits in goat.
生长抑素（SST）及其受体（SSTR1-5）似乎在影响生长、肥胖和营养吸收的许多代谢系统的中枢调节中起着重要作用。在这项研究中，我们研究了山羊 SST 和 SSTR1 基因内的多态性，并确定了它们与生长性状的关系。由于没有山羊 SST 和 SSTR1 基因的序列信息，我们探索了它们的 DNA 序列和基因组组织。山羊 SST 基因由两个外显子组成，转录成含有 351 个碱基对的 mRNA，编码 116 个氨基酸的蛋白质。其蛋白质序列与牛、人类和小鼠的相应同源物具有高度相似性（97-99%）。我们还克隆并测序了一个包含山羊 SSTR1 基因主要编码区和 3'UTR 的 1.2kb DNA 片段。然后，我们通过 PCR-SSCP 和 DNA 测序方法在来自四个品种的 459 只山羊中检测了这些确定序列中的多态性。在山羊 SST 基因的内含子 1 中发现了四个 SNP（GU014693：g.647T>C、GU014693：g.844A>C、GU014693：g.970T>C、GU014693：g.1039T>A），它们作为两个单倍型（T-A-T-T 和 C-C-C-A）分离，并与体长和体高相关（P<0.05）。在山羊 SSTR1 基因中发现了两个 SNP（GU014695：g.801 C>T、GU014695：g.948 C>T）。GU014695:801 C>T 的三种基因型与体长、体高和胸围显著相关（P<0.05）。这些结果表明，山羊 SST 和 SSTR1 基因是影响山羊生长性状的候选基因。
