Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Folia Microbiol (Praha). 2009 Nov;54(6):538-44. doi: 10.1007/s12223-009-0078-6. Epub 2010 Feb 7.
Fifty-five strains of enterococci isolated from the piglet intestine were characterized in vitro for probiotic activity. Identification of the isolates revealed Enterococcus faecium as the predominant species (84 %). Forty strains (73 %) were found to produce bacteriocin-like substances (only into solid media) with activity almost only toward Gram-positive genera. Thirty-eight % of strains were resistant to tetracycline, 27 % to chloramphenicol, 18 % to erythromycin and 16 % to vancomycin. In addition to control of strain safety, 6 % of isolates were beta-hemolytic and 16 % produced gelatinase. Seven strains selected for further probiotic assays exhibited sufficient survival rate at pH 3.0 after 3 h, in the presence of 1 % ox-bile and lysozyme after 1 d (over 107 CFU/mL in all tests). The adhesion of tested strains to porcine and human intestinal mucus was found in a similar range (1.4-14.0 % and 1.4-17.6 %, respectively). In accordance with current research effort to use and/or to combine various health promoting substances, the sensitivity of all isolates toward plant extracts and toward bacteriocins produced by animal and environmental strains was determined. All enterococci were sensitive toward oregano and sage extracts and toward one (E. faecium EF55--chicken isolate, activity of 25 600 AU/mL) of ten bacteriocin substances. It means that a similar anti-enterococcal potential of some bacteriocin substances may be observed as for certain plant extracts.
从仔猪肠道中分离出的 55 株肠球菌在体外进行了益生菌活性特征分析。分离物的鉴定表明，屎肠球菌是主要的物种（84%）。发现 40 株（73%）产生细菌素样物质（仅在固体培养基中），其活性几乎仅针对革兰氏阳性属。38%的菌株对四环素耐药，27%对氯霉素耐药，18%对红霉素耐药，16%对万古霉素耐药。除了控制菌株的安全性外，6%的分离物具有β-溶血活性，16%的分离物产生明胶酶。在 pH 值为 3.0 的条件下，选择了 7 株进一步进行益生菌检测的菌株，在 1%牛胆盐和溶菌酶存在的情况下，经过 1 天的时间后，存活率达到 3 小时后仍能保持足够的存活率（所有试验均超过 107 CFU/mL）。测试菌株对猪和人肠黏液的黏附能力在相似范围内（分别为 1.4-14.0%和 1.4-17.6%）。根据目前使用和/或结合各种促进健康的物质的研究工作，确定了所有分离株对植物提取物和动物及环境分离株产生的细菌素的敏感性。所有肠球菌对牛至和鼠尾草提取物以及一种（E. faecium EF55--鸡分离株，活性为 25600 AU/mL）的十种细菌素物质均敏感。这意味着某些细菌素物质可能具有与某些植物提取物相似的抗肠球菌潜力。
