Scharek L, Guth J, Reiter K, Weyrauch K D, Taras D, Schwerk P, Schierack P, Schmidt M F G, Wieler L H, Tedin K
Institute of Immunology and Molecular Biology, Philippstrasse 13, D-10115 Berlin, Germany.
Vet Immunol Immunopathol. 2005 May 1;105(1-2):151-61. doi: 10.1016/j.vetimm.2004.12.022.
The influence of the probiotic bacterium Enterococcus faecium SF68 on the immune system and the intestinal colonization of pigs were determined in a feeding experiment with sows and piglets. Mucosal immunity of the developing piglets was monitored by isolation and detection of intestinal lymphocyte cell populations from the proximal jejunal epithelium and the continuous Peyers patches by the use of flow cytometry. The levels of intestinal IgA in both groups of piglets were compared, as well as total IgG in the serum of sows and piglets. Feces of the sows and intestinal contents of the piglets were taken for determination of total anaerobe and coliform bacterial counts in both probiotic and control groups. Villus length and depth of the crypts were measured in the jejunum of sacrificed piglets to monitor the development of the intestinal mucosal surface amplification. Total serum IgG of the sows appeared to be unaffected. Piglets of both groups showed similar IgG levels up to 5 weeks after birth with a slight tendency toward lower values in the probiotic group. At an age of 8 weeks the total IgG levels of the probiotic animals were significantly lower (p<0.01). No differences were observed in the populations of CD4+ and CD8+ T cells in the Peyers patches. However, the levels of cytotoxic T cells (CD8+) in the jejunal epithelium of piglets of the probiotic group were significantly reduced. The depth of the jejunal crypts and length of the villi were similar in both groups, suggesting the relative T-cell population differences were not due to alterations in the epithelial cell numbers. The total anaerobe and coliform bacterial populations were not significantly affected by the probiotic treatment, either in sows or in the piglets. However, a remarkable decline in the frequency of beta-haemolytic and O141 serovars of Escherichia coli was observed in the intestinal contents of probiotic piglets, suggesting an explanation for the reduction in cytotoxic T-cell populations.
在一项针对母猪和仔猪的饲养实验中，测定了益生菌粪肠球菌SF68对猪免疫系统和肠道定植的影响。通过使用流式细胞术从空肠近端上皮和连续的派伊尔结中分离并检测肠道淋巴细胞群体，监测发育中仔猪的黏膜免疫。比较了两组仔猪肠道IgA水平以及母猪和仔猪血清中的总IgG水平。采集母猪粪便和仔猪肠道内容物，测定益生菌组和对照组中的总厌氧菌和大肠菌群细菌数量。测量处死仔猪空肠的绒毛长度和隐窝深度，以监测肠道黏膜表面增殖的发育情况。母猪的血清总IgG似乎未受影响。两组仔猪在出生后5周内的IgG水平相似，益生菌组有轻微的降低趋势。在8周龄时，益生菌组动物的总IgG水平显著降低（p<0.01）。在派伊尔结中，CD4+和CD8+ T细胞群体未观察到差异。然而，益生菌组仔猪空肠上皮中的细胞毒性T细胞（CD8+）水平显著降低。两组空肠隐窝深度和绒毛长度相似，表明相对T细胞群体差异并非由于上皮细胞数量的改变。益生菌处理对母猪或仔猪的总厌氧菌和大肠菌群数量均无显著影响。然而，在益生菌组仔猪的肠道内容物中，观察到大肠杆菌β溶血和O141血清型的频率显著下降，这为细胞毒性T细胞群体的减少提供了解释。
