Department of Biochemistry, University of Porto, Portugal.
Cell Mol Biol Lett. 2010 Jun;15(2):234-41. doi: 10.2478/s11658-010-0006-4. Epub 2010 Feb 8.
There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer's and Parkinson's diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.
人们越来越关注饮食治疗策略，以对抗中枢神经系统（CNS）中氧化应激引起的损伤，氧化应激与许多病理生理过程有关，包括阿尔茨海默病、帕金森病和脑血管疾病。由于缺乏这些化合物进入中枢神经系统的能力的信息，与酚类神经保护相关的机制的确定一直受到阻碍。本研究的目的是评估类黄酮跨 RBE-4 细胞（大鼠脑毛细血管内皮细胞的永生化细胞系）的跨膜转运以及乙醇对这种转运的影响。使用 HPLC-DAD（二极管阵列检测器）检测和定量研究样品（基底外侧介质）中的所有酚类化合物。所有测试的类黄酮（儿茶素、槲皮素和矢车菊素-3-葡萄糖苷）都以时间依赖性方式穿过 RBE-4 细胞。这种转运不受 0.1%乙醇的存在影响。总之，测试的类黄酮能够穿过这种血脑屏障模型。
