Czeczot Hanna, Majewska Monika, Skrzycki Michał, Podsiad Małgorzata
Katedra i Zakład Biochemii Warszawskiego Uniwersytetu Medycznego.
Wiad Lek. 2009;62(2):87-92.
Since one of the many proposed factors in the pathogenesis of acute and chronic pancreatitis is oxidative stress, the aim of the research was evaluation of antioxidant defense mechanisms, with particular emphasis on the role of reduced glutathione and GSH-dependent enzymes.
The study involved a group of 35 patients with pancreatitis treated at the Clinic of General Surgery and Transplantation Medical University of Warsaw in the period from 2005 to 2007. This group consisted of 20 patients with mild symptoms (edema) of the form of acute pancreatitis and 15 patients with chronic pancreatitis, short duration of the disease. In all patients with acute and chronic pancreatitis qualified for the study were measured in serum markers of oxidative stress: concentrations of reactive thiobarbituric acid (TBARS), which determines the level of lipid peroxidation and reduced levels of glutathione (GSH) and activity of antioxidant enzymes: total glutathione peroxidase (cal. GSHPx), glutathione S-transferase (GST) and glutathione reductase (GSHR).
We found increased lipid peroxidation level, decreased level of GSH, and changes in activity of GSH-dependent enzymes in blood serum of patients with acute and chronic pancreatitis, compared to blood serum from healthy persons.
Obtained results indicate participation of oxidative stress in pathogenesis of those diseases, and systemic impairment of antioxidative mechanisms.
由于急性和慢性胰腺炎发病机制中众多因素之一是氧化应激，本研究的目的是评估抗氧化防御机制，尤其着重于还原型谷胱甘肽和谷胱甘肽依赖性酶的作用。
该研究纳入了2005年至2007年期间在华沙医科大学普通外科和移植科接受治疗的35例胰腺炎患者。该组包括20例急性胰腺炎轻症（水肿型）患者和15例病程较短的慢性胰腺炎患者。对所有纳入研究的急性和慢性胰腺炎患者测定了氧化应激血清标志物：反应性硫代巴比妥酸（TBARS）浓度，其决定脂质过氧化水平；还原型谷胱甘肽（GSH）水平降低；以及抗氧化酶活性：总谷胱甘肽过氧化物酶（计算值GSHPx）、谷胱甘肽S-转移酶（GST）和谷胱甘肽还原酶（GSHR）。
与健康人血清相比，我们发现急性和慢性胰腺炎患者血清中脂质过氧化水平升高、GSH水平降低以及谷胱甘肽依赖性酶活性发生变化。
获得的结果表明氧化应激参与了这些疾病的发病机制，且抗氧化机制存在全身性损害。
