两种不同微生物试剂盒检测牙周病病原体的比较。
Comparison of two different microbiological test kits for detection of periodontal pathogens.
机构信息
Department of Operative Dentistry, Section of Periodontology, University Hospital Heidelberg, Heidelberg, Germany.
出版信息
Acta Odontol Scand. 2010 Mar;68(2):115-21. doi: 10.3109/00016350903514848.
OBJECTIVE
The aim of this study was to compare the outcome of two different microbiological tests for detection of Aggregatibacter actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.), Tannerella forsythia (T.f.) and Treponema denticola (T.d.).
MATERIAL AND METHODS
A total of 69 adult patients with severe chronic (sCP) or aggressive periodontitis (AgP) participated in the study. Microbiological samples were examined for A.a., P.g., T.f. and T.d. using an RNA probe test (PADO) and a real-time polymerase chain reaction test (MERI).
RESULTS
For all periodontal pathogens under investigation, the median bacterial counts detected with PADO were smaller compared to those detected with MERI. P.g., T.f. and T.d. could be found in the majority of all patients with both tests. With MERI, A.a. was detected more often (24.6%) than with PADO (18.8%). Only 10.1% of the patients tested positive for A.a. with both tests.
CONCLUSION
Both tests showed a high percentage of agreement for P.g., T.f. and T.d., but exhibited marked differences in the detection of A.a.
目的
本研究旨在比较两种不同的微生物检测方法（聚合酶链反应法和 RNA 探针法）在检测伴放线放线杆菌（A.a.）、牙龈卟啉单胞菌（P.g.）、福赛坦纳菌（T.f.）和牙髓密螺旋体（T.d.）时的效果。
材料与方法
共有 69 名患有严重慢性（sCP）或侵袭性牙周炎（AgP）的成年患者参与了本研究。使用 RNA 探针法（PADO）和实时聚合酶链反应法（MERI）对 A.a.、P.g.、T.f. 和 T.d.进行微生物样本检测。
结果
对于所有研究的牙周病原体，PADO 检测到的细菌计数中位数均小于 MERI 检测到的细菌计数中位数。两种检测方法均能在大多数患者中检测到 P.g.、T.f. 和 T.d. 用 MERI 检测到 A.a.的频率（24.6%）高于用 PADO 检测到的频率（18.8%）。仅 10.1%的患者用两种检测方法均检测出 A.a.阳性。
结论
两种检测方法在检测 P.g.、T.f. 和 T.d. 时均具有较高的一致性，但在检测 A.a.时存在明显差异。
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