Meyer-Bäumer Amelie, Eick Sigrun, Mertens Christian, Uhlmann Lorenz, Hagenfeld Daniel, Eickholz Peter, Kim Ti-Sun, Cosgarea Raluca
Section of Periodontology, Department of Conservative Dentistry, Clinic for Oral, Dental and Maxillofacial Diseases, University Hospital Heidelberg, Heidelberg, Germany.
J Clin Periodontol. 2014 Jul;41(7):662-72. doi: 10.1111/jcpe.12255. Epub 2014 May 25.
To assess the association between presence of periodontal pathogens and recurrence of disease in patients with aggressive periodontitis (AgP) after active periodontal therapy (APT) and further influencing factors.
MATERIAL & METHODS: Microbiological samples were taken from 73 patients with AgP 5-17 years after APT at 292 sites (deepest site per quadrant). Real-time polymerase chain reactions were used to detect the periodontal pathogens Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. Uni- and multivariate analyses evaluated the associations between pathogens and recurrence of disease, smoking and adjunctive antibiotic therapy.
At re-examination A. actinomycetemcomitans could be detected in six patients (8.2%), P. gingivalis in 24 (32.9%), T. forsythia in 31 (42.5%) and T. denticola in 35 (48.0%). Increased levels of T. forsythia and T. denticola at re-examination were significantly associated with recurrence of disease in multivariate analyses (OR: 12.72, p < 0.001; OR 5.55, p = 0.002 respectively). Furthermore, high counts of T. denticola were found in patients with increased percentage of sites with clinical attachment levels (CAL) ≥ 6 mm compared to those with low counts (13.8% versus 3.2%, p = 0.005).
In patients with recurrence of disease T. forsythia and T. denticola were detected more frequently and in higher counts. Furthermore, T. denticola was found more frequently in patients with increased CAL.
评估侵袭性牙周炎（AgP）患者在进行积极牙周治疗（APT）后牙周病原体的存在与疾病复发之间的关联以及其他影响因素。
在APT后5 - 17年，从73例AgP患者的292个部位（每个象限最深部位）采集微生物样本。采用实时聚合酶链反应检测牙周病原体伴放线聚集杆菌、牙龈卟啉单胞菌、福赛坦氏菌和具核梭杆菌。单因素和多因素分析评估病原体与疾病复发、吸烟及辅助抗生素治疗之间的关联。
复查时，6例患者（8.2%）检测到伴放线聚集杆菌，24例（32.9%）检测到牙龈卟啉单胞菌，31例（42.5%）检测到福赛坦氏菌，35例（48.0%）检测到具核梭杆菌。多因素分析显示，复查时福赛坦氏菌和具核梭杆菌水平升高与疾病复发显著相关（OR分别为12.72，p < 0.001；OR为5.55，p = 0.002）。此外，与具核梭杆菌计数低的患者相比，临床附着丧失（CAL）≥6 mm部位百分比增加的患者中具核梭杆菌计数高（13.8%对3.2%，p = 0.005）。
疾病复发的患者中，福赛坦氏菌和具核梭杆菌的检出频率更高且数量更多。此外，CAL增加的患者中具核梭杆菌的检出频率更高。
