Barrett Julie A, Siegel Jay A, Goodpaster John V
Department of Chemistry, Butler University, Indianapolis, IN, USA.
J Forensic Sci. 2010 Mar 1;55(2):323-33. doi: 10.1111/j.1556-4029.2009.01306.x. Epub 2010 Feb 5.
Current protocols for examining hair do not attempt to differentiate hair color using instrumental analysis. In this study, hair samples treated with 55 different red hair dyes were analyzed using UV-visible microspectrophotometry between 200 and 700 nm. Using air as a background reference gave the best results, although mounting media such as glycerin could also be used. The contribution of the hair substrate is predominantly observed in the range of 300-400 nm while the dye peak is evident in the range of 425-550 nm. It was found that the presence of hair dye reduces the overall intrasample variability of the hair color. In addition, visual inspection and spectral interpretation showed that dyed hair exhibits distinct and discernable shades. The color of all samples was stable during storage and while all hair dyes fade with washing, significant fading of the color was only evident after daily washing for 3 weeks.
当前的毛发检测方案并未尝试通过仪器分析来区分头发颜色。在本研究中，使用紫外可见显微分光光度法在200至700纳米之间对用55种不同红色染发剂处理过的头发样本进行了分析。以空气作为背景参考得到的结果最佳，不过甘油等 mounting media 也可使用。毛发基质的贡献主要在300 - 400纳米范围内观察到，而染料峰值在425 - 550纳米范围内很明显。研究发现，染发剂的存在降低了头发颜色样本内的总体变异性。此外，目视检查和光谱解读表明，染色后的头发呈现出明显且可辨别的色调。所有样本的颜色在储存期间保持稳定，并且虽然所有染发剂都会随着洗涤而褪色，但只有在每日洗涤3周后颜色才会明显褪色。
