磁珠文库筛选更轻松。
On-bead library screening made easier.
作者信息
Pei Dehua
机构信息
Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA.
出版信息
Chem Biol. 2010 Jan 29;17(1):3-4. doi: 10.1016/j.chembiol.2010.01.003.
Abstract
In this issue of Chemistry & Biology, Astle et al. report the rapid identification of the most active hits from a large one-bead-one-compound peptoid library by magnetic sorting and without the need for labor-intensive resynthesis of the hits.
摘要
在本期《化学与生物学》中，阿斯尔等人报告了通过磁分选从一个大型单珠单化合物类肽文库中快速鉴定出活性最高的命中物，且无需对命中物进行费力的重新合成。
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本文引用的文献
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2
Single bead labeling method for combining confocal fluorescence on-bead screening and solution validation of tagged one-bead one-compound libraries.用于结合共聚焦荧光微珠筛选和标记的单珠单化合物文库溶液验证的单珠标记方法。
Chem Biol. 2009 Jul 31;16(7):724-35. doi: 10.1016/j.chembiol.2009.06.011.
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On-bead screening of combinatorial libraries: reduction of nonspecific binding by decreasing surface ligand density.组合文库的珠上筛选：通过降低表面配体密度减少非特异性结合
J Comb Chem. 2009 Jul-Aug;11(4):604-11. doi: 10.1021/cc9000168.
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High-throughput sequencing of peptoids and peptide-peptoid hybrids by partial edman degradation and mass spectrometry.通过部分埃德曼降解和质谱法对类肽及肽-类肽杂合物进行高通量测序。
J Comb Chem. 2009 Mar 9;11(2):294-302. doi: 10.1021/cc8001734.
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Cleavable hydrophilic linker for one-bead-one-compound sequencing of oligomer libraries by tandem mass spectrometry.用于通过串联质谱对寡聚体文库进行单珠单化合物测序的可裂解亲水性连接子。
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