Miller Stephen D, Karpus William J, Davidson Todd Scott
Northwestern University, Evanston, Illinois.
NIAID, NIH, Bethesda, Maryland.
Curr Protoc Immunol. 2010 Feb;Chapter 15:15.1.1-15.1.20. doi: 10.1002/0471142735.im1501s88.
This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. A protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.
本单元详细介绍了使用来自两种主要髓鞘蛋白（蛋白脂蛋白（PLP）和髓鞘碱性蛋白（MBP））的完整蛋白质或肽，在SJL小鼠品系中主动诱导和过继转移实验性自身免疫性脑脊髓炎（EAE）所需的材料和方法。还描述了纯化PLP和MBP所需的详细材料和方法。包括了在EAE小鼠中分离中枢神经系统浸润淋巴细胞的方案。对于在其他小鼠品系中有效诱导主动或过继性EAE，可能需要对指定方案进行修改。
