MicroRNA155 在小鼠 TNBS 诱导的结肠炎激活的 CD4(+) T 细胞中诱导产生。
MicroRNA155 is induced in activated CD4(+) T cells of TNBS-induced colitis in mice.
机构信息
Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Second Road, Shanghai 200025, China.
出版信息
World J Gastroenterol. 2010 Feb 21;16(7):854-61. doi: 10.3748/wjg.v16.i7.854.
AIM
To investigate the expression of microRNA155 (miRNA155) in trinitrobenzene sulphonic acid (TNBS)-induced colitis and the relationship between miRNA155 and tumor necrosis factor (TNF) expressions.
METHODS
In TNBS colitis mice, miRNA155 and TNF mRNA expressions were measured in colons and CD4(+) T cells of draining lymph nodes (LNs). CD4(+) T cells were cultured in vitro with or without anti-CD3/CD28 antibody, and the expressions of miRNA155 and TNF mRNA in cells and TNF concentration in culture media were examined.
RESULTS
miRNA155 and TNF mRNA expressions in colons and in cells of LNs were significantly increased in TNBS colitis compared with controls. In TNBS colitis, miRNA155 and TNF mRNA expressions in CD4(+) T cells of LNs and TNF concentration in CD4(+) T cells culture media increased compared with controls. When cultured with anti-CD3/CD28 antibody, miRNA155 and TNF mRNA expressions in CD4(+) T cells and TNF concentration in the CD4(+) T cells culture media were significantly higher than those cultured without anti-CD3/CD28 antibody. Following analysis using the Pearson's correlation coefficient, miRNA155 expression had a significant positive correlation with either TNF mRNA expression in CD4(+) T cells (r = 0.860, P < 0.05) or TNF concentration in CD4(+) T cells culture media (r = 0.892, P < 0.05).
CONCLUSION
miRNA155 is induced in colons and activated CD4(+) T cells in TNBS colitis, and the levels of miRNA155 and TNF expressions have a significant positive correlation.
目的
研究三硝基苯磺酸（TNBS）诱导结肠炎中 microRNA155（miRNA155）的表达及其与肿瘤坏死因子（TNF）表达的关系。
方法
在 TNBS 结肠炎小鼠中，测量结肠和引流淋巴结（LN）中 CD4+T 细胞中 miRNA155 和 TNF mRNA 的表达。体外培养 CD4+T 细胞，并用或不用抗 CD3/CD28 抗体，检测细胞中 miRNA155 和 TNF mRNA 的表达及细胞培养上清液中 TNF 浓度。
结果
与对照组相比，TNBS 结肠炎中结肠和 LN 中 CD4+T 细胞中 miRNA155 和 TNF mRNA 的表达显著增加。在 TNBS 结肠炎中，与对照组相比，LN 中 CD4+T 细胞中的 miRNA155 和 TNF mRNA 的表达以及 CD4+T 细胞培养上清液中的 TNF 浓度增加。当用抗 CD3/CD28 抗体培养时，CD4+T 细胞中的 miRNA155 和 TNF mRNA 的表达以及 CD4+T 细胞培养上清液中的 TNF 浓度明显高于未用抗 CD3/CD28 抗体培养的细胞。通过 Pearson 相关系数分析，miRNA155 表达与 CD4+T 细胞中的 TNF mRNA 表达（r = 0.860，P < 0.05）或 CD4+T 细胞培养上清液中的 TNF 浓度（r = 0.892，P < 0.05）呈显著正相关。
结论
在 TNBS 结肠炎中，miRNA155 在结肠和激活的 CD4+T 细胞中被诱导，miRNA155 和 TNF 的表达水平呈显著正相关。
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