Rodriguez Antony, Vigorito Elena, Clare Simon, Warren Madhuri V, Couttet Philippe, Soond Dalya R, van Dongen Stijn, Grocock Russell J, Das Partha P, Miska Eric A, Vetrie David, Okkenhaug Klaus, Enright Anton J, Dougan Gordon, Turner Martin, Bradley Allan
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
Science. 2007 Apr 27;316(5824):608-11. doi: 10.1126/science.1139253.
MicroRNAs are a class of small RNAs that are increasingly being recognized as important regulators of gene expression. Although hundreds of microRNAs are present in the mammalian genome, genetic studies addressing their physiological roles are at an early stage. We have shown that mice deficient for bic/microRNA-155 are immunodeficient and display increased lung airway remodeling. We demonstrate a requirement of bic/microRNA-155 for the function of B and T lymphocytes and dendritic cells. Transcriptome analysis of bic/microRNA-155-deficient CD4+ T cells identified a wide spectrum of microRNA-155-regulated genes, including cytokines, chemokines, and transcription factors. Our work suggests that bic/microRNA-155 plays a key role in the homeostasis and function of the immune system.
微小RNA是一类小RNA，越来越被认为是基因表达的重要调节因子。尽管哺乳动物基因组中存在数百种微小RNA，但针对其生理作用的遗传学研究尚处于早期阶段。我们已经表明，缺乏bic/微小RNA-155的小鼠存在免疫缺陷，并表现出肺部气道重塑增加。我们证明了bic/微小RNA-155对B淋巴细胞、T淋巴细胞和树突状细胞功能的必要性。对缺乏bic/微小RNA-155的CD4+ T细胞进行转录组分析，确定了广泛的微小RNA-155调节基因，包括细胞因子、趋化因子和转录因子。我们的工作表明，bic/微小RNA-155在免疫系统的稳态和功能中起关键作用。
