Ptaszyńska-Sarosiek Iwona, Niemcunowicz-Janica Anna, Pepiński Witold, Skawrońska Małgorzata, Koc-Zórawska Ewa, Janica Jerzy
Zakład Medycyny Sadowej Akademii Medycznej w Białymstoku, ul. Kilińskiego 1, 15-230 Białystok.
Ann Acad Med Stetin. 2007;53 Suppl 2:28-32; discussion 32.
Y-STRs are of an importance in forensic genetics in cases of selective genotyping of male haplotypes and/or determination of a number of perpetrators. Experimental vaginal swabs containing semen were collected to evaluate the effect of storage conditions and male/female DNA ratio on the efficiency of amplification of Powerplex Y system alleles. The specimens were collected on disposable devices from 20 volunteer pairs of sexual partners. The swabs were stored on selected supports in different temperature conditions. DNA templates were extracted using Chelex and quantitated spectrophotometrically. Powerplex Y system (Promega) ABI Prism 310 (Applera) were used to obtain Y-STR profiles. Vaginal swabs airdryed at room temperature were found the best source of DNA for male identification. Full Y-STR haplotypes were obtained in male/female mixtures prepared in proportion of 1 : 1000.
Y染色体短串联重复序列（Y-STRs）在法医遗传学中具有重要意义，可用于男性单倍型的选择性基因分型和/或确定犯罪者数量的案件。收集含有精液的实验性阴道拭子，以评估储存条件和男女DNA比例对Powerplex Y系统等位基因扩增效率的影响。从20对自愿性伴侣中，在一次性装置上采集标本。拭子在选定的载体上于不同温度条件下储存。使用Chelex提取DNA模板，并通过分光光度法定量。使用Powerplex Y系统（Promega公司）和ABI Prism 310（Applera公司）获得Y-STR图谱。发现室温下风干的阴道拭子是用于男性身份鉴定的最佳DNA来源。在按1:1000比例制备的男女混合物中获得了完整的Y-STR单倍型。
