Microbiology Service, University Hospital of Valme, Seville-Cádiz Road, Seville, Spain.
Med Mycol. 2010 Sep;48(6):858-61. doi: 10.3109/13693781003586943.
We performed Etest and broth microdilution (BMD) susceptibility testing of caspofungin, micafungin and anidulafungin against 67 clinical isolates of Aspergillus spp. and 10 Fusarium spp. Minimal effective concentrations (MECs) by BMD were read after 24 h of incubation at 35 degrees C and Etest MICs were read at 24 and 48 h. MECs < or =0.25 mg/l were obtained with caspofungin for all Aspergillus spp. tested but Etest MICs were < or =1 mg/l at 24 h. The agreement between caspofungin Etest and broth microdilution was good for all Aspergillus species tested (range 82.4-100%) except for A. niger and A. glaucus at 24 h of incubation. Micafungin and anidulafungin MEC and MIC results were lower than those of caspofungin (< or =0.015 mg/l) at 24 and 48 h for all Aspergillus tested. The agreement between the methods was excellent (100%) for micafungin and anidulafungin for all Aspergillus species tested. The three echinocandins were inactive against all isolates of Fusarium spp. showing MECs and MICs >8 mg/l. The Etest method could be a suitable procedure to test the susceptibility of most Aspergillus species to caspofungin, micafungin and anidulafungin; the best agreement was at 24 h.
我们对 67 株临床分离的曲霉菌属和 10 株镰刀菌属进行了卡泊芬净、米卡芬净和阿尼芬净的 Etest 和肉汤微量稀释法（BMD）药敏试验。在 35°C 孵育 24 小时后读取 BMD 的最小有效浓度（MEC），并在 24 和 48 小时读取 Etest MIC。对于所有测试的曲霉菌属，卡泊芬净的 MEC<或=0.25mg/l，但在 24 小时时 Etest MIC<或=1mg/l。对于所有测试的曲霉菌属，卡泊芬净 Etest 和肉汤微量稀释法之间的一致性良好（范围 82.4-100%），除了在 24 小时孵育时的黑曲霉和棒曲霉。米卡芬净和阿尼芬净的 MEC 和 MIC 结果在 24 和 48 小时时均低于卡泊芬净（<或=0.015mg/l），对于所有测试的曲霉菌属。对于所有测试的曲霉菌属，米卡芬净和阿尼芬净的方法之间的一致性非常好（100%）。三种棘白菌素对所有镰刀菌属的分离株均无活性，显示 MEC 和 MIC>8mg/l。Etest 方法可用于测试大多数曲霉菌属对卡泊芬净、米卡芬净和阿尼芬净的敏感性；最佳一致性是在 24 小时。
