Toy Harun, Camuzcuoglu Hakan, Arioz Dagistan Tolga, Kurt Sefa, Celik Hakim, Aksoy Nurten
Department of Obstetrics and Gynecology, School of Medicine, Harran University, Sanliurfa, Turkey.
J Obstet Gynaecol Res. 2009 Dec;35(6):1047-53. doi: 10.1111/j.1447-0756.2009.01063.x.
To compare the levels of serum prolidase activity and oxidative stress markers, including total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), and total free sulfhydryl (-SH) in healthy pregnant women and pregnant women with intrauterine growth restricted infants.
Serum samples were obtained from 18 healthy third trimester pregnancies and 14 pregnancies complicated by fetal growth restriction (FGR). The criteria for FGR were clinical evidence of suboptimal growth, ultrasonographic demonstration of deviation from normal percentiles of growth, and birthweight under the 10th percentile. We spectrophotometrically measured serum prolidase activity, TAC, TOS, and -SH levels. Oxidative stress was determined from total antioxidant capacity and total oxidant status measurement and calculation of the oxidative stress index.
Serum TAC and -SH levels were significantly lower in the FGR group than in the healthy control group (P = 0.001, P < 0.001, respectively), whereas TOS level, OSI value, and prolidase activity were significantly higher (P = 0.003, P < 0.001, P < 0.001, respectively). Prolidase activity was positively correlated with TOS and OSI values (rho = 0.552, P = 0.041 and rho = 0.635, P = 0.015, respectively) and negatively correlated with TAC and -SH levels (rho = -0.578, P = 0.030 and rho = -0.622, P = 0.018, respectively).
The present study shows that serum prolidase activity and oxidative stress are significantly associated with the presence of FGR and that the correlation between serum prolidase activity and markers of oxidative stress are represented as increased serum TOS level and decreased serum TAC and -SH levels, suggesting an association of collagen turnover and oxidative stress in vascular dysfunction.
比较健康孕妇与怀有宫内生长受限胎儿的孕妇血清脯氨酰肽酶活性及氧化应激标志物水平，包括总氧化状态（TOS）、总抗氧化能力（TAC）、氧化应激指数（OSI）和总游离巯基（-SH）。
采集18例健康孕晚期孕妇和14例合并胎儿生长受限（FGR）孕妇的血清样本。FGR的标准为生长欠佳的临床证据、超声显示生长偏离正常百分位数以及出生体重低于第10百分位数。我们采用分光光度法测定血清脯氨酰肽酶活性、TAC、TOS和-SH水平。通过总抗氧化能力和总氧化状态测量及氧化应激指数计算来确定氧化应激。
FGR组血清TAC和-SH水平显著低于健康对照组（分别为P = 0.001，P < 0.001），而TOS水平、OSI值和脯氨酰肽酶活性显著更高（分别为P = 0.003，P < 0.001，P < 0.001）。脯氨酰肽酶活性与TOS和OSI值呈正相关（分别为rho = 0.552，P = 0.041和rho = 0.635，P = 0.015），与TAC和-SH水平呈负相关（分别为rho = -0.578，P = 0.030和rho = -0.622，P = 0.018）。
本研究表明，血清脯氨酰肽酶活性和氧化应激与FGR的存在显著相关，且血清脯氨酰肽酶活性与氧化应激标志物之间的相关性表现为血清TOS水平升高、血清TAC和-SH水平降低，提示在血管功能障碍中胶原代谢与氧化应激存在关联。
