Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Virol J. 2010 Feb 10;7:33. doi: 10.1186/1743-422X-7-33.
The TAR RNA of HIV was engineered as an siRNA delivery vehicle to develop a combinatorial therapeutic approach. The TAR backbone was found to be a versatile backbone for expressing siRNAs. Upon expression in human cells, pronounced and specific inhibition of reporter gene expression was observed with TARmiR. The resulting TARmiR construct retained its ability to bind Tat and mediate RNAi. TARmiR was able to inhibit HIV gene expression as a TAR decoy and by RNA interference when challenged with infectious proviral DNA. The implications of this dual function therapeutic would be discussed.
HIV 的 TAR RNA 被设计为 siRNA 递药载体，以开发组合治疗方法。TAR 骨架被发现是表达 siRNA 的多功能骨架。在人类细胞中表达时，TARmiR 观察到明显且特异性的报告基因表达抑制。所得的 TARmiR 构建体保留了结合 Tat 和介导 RNAi 的能力。TARmiR 能够作为 TAR 诱饵抑制 HIV 基因表达，并在受到感染性前病毒 DNA 挑战时通过 RNA 干扰抑制 HIV 基因表达。将讨论这种双重功能治疗的意义。
