对氯苯丙氨酸减少大鼠中缝核 5-羟色胺能细胞的性别和区域性差异。
Sex and regional differences in decrease of serotonin-immunoreactive cells by parachlorophenylalanine in rat raphe nuclei.
机构信息
Neuroendocrinology, Department of Human Behavior and Environment Sciences, School of Human Sciences, Waseda University, 2-579-15 Mikajima, Tokorozawa, Saitama 359-1192, Japan.
出版信息
Neurosci Res. 2010 May;67(1):33-9. doi: 10.1016/j.neures.2010.02.002. Epub 2010 Feb 6.
To determine sex and regional differences in the properties of serotonin (5-HT) neurons of the raphe nuclei, the responsiveness to parachlorophenylalanine (PCPA) of 5-HT neurons in the dorsal and median raphe nuclei (DR and MR) and the nucleus raphe magnus (RMg) was analyzed by counting 5-HT-immunoreactive (5-HT-ir) cells. Gonadectomized male (OCX) and female (OVX) rats were treated with 100mg/kg b.wt PCPA or saline daily for 4 days. The brains were removed and fixed one day after the last injection. Frozen sections were stained with serotonin antibody and the numbers of 5-HT-ir cells in the raphe nuclei were counted. As a result, in female rats, the densities of 5-HT-ir cells in these 3 raphe nuclei were almost the same when compared the PCPA-treated and saline-treated groups. On the other hand, in male rats, the densities of 5-HT-ir cells in the DR and MR of PCPA-treated rats were lower than in saline-treated rats. In the male RMg, no difference was seen. These results suggest that responsiveness of 5-HT neurons to PCPA in the DR and MR, but not in the RMg, were sexually dimorphic.
为了确定中缝核 5-羟色胺（5-HT）神经元的性别和区域差异，通过计数 5-HT 免疫反应（5-HT-ir）细胞来分析背侧和中缝核（DR 和 MR）以及中缝核巨细胞核（RMg）中 5-HT 神经元对对氯苯丙氨酸（PCPA）的反应性。去势雄性（OCX）和雌性（OVX）大鼠每天用 100mg/kg b.wt PCPA 或生理盐水处理 4 天。最后一次注射后一天取出大脑并固定。用血清素抗体对冷冻切片进行染色，并计数中缝核中 5-HT-ir 细胞的数量。结果表明，与 PCPA 处理组相比，雌性大鼠这 3 个中缝核中的 5-HT-ir 细胞密度几乎相同。另一方面，在雄性大鼠中，PCPA 处理组 DR 和 MR 中的 5-HT-ir 细胞密度低于生理盐水处理组。在雄性 RMg 中，没有差异。这些结果表明，DR 和 MR 中 5-HT 神经元对 PCPA 的反应性存在性别二态性，但 RMg 中则没有。
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