Department of Rheumatology, Level 4 Eleanor Harrald Building, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia 5042, Australia.
Postgrad Med J. 2010 Feb;86(1012):79-82. doi: 10.1136/pgmj.2009.089656.
To determine the prevalence and diagnostic utility of monospecific anti-Ro52 (defined as an immune response against Ro-52 antigen in the absence of reactivity to Ro-60 antigen) reactivity in selected autoimmune diseases.
Stored diagnostic non-consecutive serum samples obtained from patients with systemic lupus erythematosus (SLE), primary Sjögren's syndrome (pSS), systemic sclerosis, idiopathic inflammatory myopathies (IIM), rheumatoid arthritis, primary biliary cirrhosis and mixed essential cryoglobulinaemia were analysed by line immunoassay to detect the presence of anti-Ro52 and other autoantibodies.
Monospecific anti-Ro52 reactivity was found in 51 (12.7%) of the 402 samples tested. Anti-Ro52 was the most common serological marker in patients with IIM (35/147, 23.8%) and co-occurred with anti-Jo1 (10/18, 55.6%; p=0.02). The prevalence of anti-Ro52 reactivity was significantly more than anti-Ro60 reactivity in patients with IIM, systemic sclerosis, primary biliary cirrhosis, mixed essential cryoglobulinemia and pSS. The mean signal intensity of anti-Ro52 reactivity was significantly higher in pSS than SLE and associated with rheumatoid factor positivity. The mean signal intensity of anti-Ro52 correlated with anti-Ro60 and anti-La in pSS and SLE.
Monospecific anti-Ro52 reactivity is not disease specific but may be of importance in patients with IIM. Furthermore, as anti-Ro52 reactivity is more prevalent than anti-Ro60 reactivity in certain autoimmune conditions, specific testing for their distinction in clinical practice is recommended.
确定在选定的自身免疫性疾病中，抗 Ro52 单特异性反应（定义为针对 Ro-52 抗原的免疫反应，而不存在对 Ro-60 抗原的反应性）的流行率和诊断效用。
通过线免疫分析检测系统性红斑狼疮（SLE）、原发性干燥综合征（pSS）、系统性硬化症、特发性炎性肌病（IIM）、类风湿关节炎、原发性胆汁性肝硬化和混合性必需性冷球蛋白血症患者的储存诊断性非连续血清样本中是否存在抗 Ro52 和其他自身抗体。
在 402 个测试样本中，有 51 个（12.7%）存在抗 Ro52 单特异性反应。抗 Ro52 是 IIM 患者最常见的血清学标志物（35/147，23.8%），与抗 Jo1 同时存在（10/18，55.6%；p=0.02）。在 IIM、系统性硬化症、原发性胆汁性肝硬化、混合性必需性冷球蛋白血症和 pSS 患者中，抗 Ro52 反应的发生率明显高于抗 Ro60 反应。pSS 患者的抗 Ro52 反应平均信号强度明显高于 SLE，且与类风湿因子阳性相关。pSS 和 SLE 中抗 Ro52 反应的平均信号强度与抗 Ro60 和抗 La 相关。
抗 Ro52 单特异性反应不是疾病特异性的，但可能对 IIM 患者很重要。此外，由于抗 Ro52 反应在某些自身免疫性疾病中的发生率高于抗 Ro60 反应，因此建议在临床实践中进行特异性检测以区分它们。
