Dunn M J, Rose M L, Latif N, Bradd S, Lovegrove C, Seymour C, Pomerance A, Yacoub M H
Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, United Kingdom.
Transplantation. 1991 Apr;51(4):806-12. doi: 10.1097/00007890-199104000-00014.
Western blotting has been used to detect antiheart antibodies in two groups of patients: two who required retransplantation for hyperacute rejection, and 22 consecutive patients, whose serum was tested at monthly intervals for three months following transplantation. Pretransplant and posttransplant serum samples were tested for IgM and IgG reactivity against the patients own heart and donor heart. In all patients the pretransplant lymphocytic crossmatch had been negative. In the two patients requiring retransplantation, both had multiple bands of strong IgM and IgG against their own heart prior to transplantation as well as antibodies against the donor heart. The study of 22 consecutive patients revealed that (1) the presence of strong antibody prior to transplantation is associated with unusually severe or frequent rejection episodes, (2) 20/22 patients made antiheart antibody following transplantation, but in 12 patients it was IgM only, and (3) most of the antiheart antibodies made posttransplant were not specific for the donor heart. Comparison of Western blotting with immunofluorescent detection of antibodies on frozen sections revealed that the Western blotting procedure is more sensitive and results are easier to interpret.
两名因超急性排斥反应需要再次移植的患者，以及22名连续患者，在移植后的三个月内每月检测一次他们的血清。对移植前和移植后的血清样本检测针对患者自身心脏和供体心脏的IgM和IgG反应性。所有患者移植前淋巴细胞交叉配型均为阴性。在两名需要再次移植的患者中，两人在移植前均有针对自身心脏的多条强IgM和IgG条带以及针对供体心脏的抗体。对22名连续患者的研究表明：（1）移植前存在强抗体与异常严重或频繁的排斥反应发作有关；（2）22名患者中有20名在移植后产生了抗心脏抗体，但其中12名患者仅产生了IgM；（3）移植后产生的大多数抗心脏抗体并非供体心脏特异性的。蛋白质印迹法与冷冻切片抗体免疫荧光检测法的比较表明，蛋白质印迹法更灵敏，结果更易于解读。
